{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:41:40Z","timestamp":1788370900685,"version":"build-2803163510"},"reference-count":8,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:00:00Z","timestamp":1597017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Labelled Comp Radiopharmac"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:p>CART (cocaine\u2010 and amphetamine\u2010regulated transcript) peptides are involved in food intake regulation, stress, and other physiological functions. Although CART peptides have been known for over 25 years, their receptor(s) have not yet been characterized. In this short review, we will summarize our previous studies, where we reported specific binding of <jats:sup>125<\/jats:sup>I\u2010CART(61\u2010102) to PC12 rat pheochromocytoma cells. Competitive binding experiments performed with mono\u2010 and di\u2010iodinated peptides and their isoforms with oxidized Met<jats:sup>67<\/jats:sup> resulted in nanomolar binding affinity. Moreover, in our previous study, CART(61\u2010102), as well as di\u2010iodinated CART(61\u2010102), have shown a strong anorexigenic effect in fasted lean mice after intracerebroventricular administration. In conclusion, from our previous studies, iodination of CART(61\u2010102) resulted in mono\u2010 and di\u2010iodinated analogs with or without oxidized Met<jats:sup>67<\/jats:sup>. All analogs revealed a high affinity to binding sites at PC12 cells and preserved biological activity.<\/jats:p>","DOI":"10.1002\/jlcr.3871","type":"journal-article","created":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T13:42:55Z","timestamp":1594993375000},"page":"61-64","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Iodination of CART(61\u2010102) peptide: Preserved binding and anorexigenic activity in mice"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9365-078X","authenticated-orcid":false,"given":"Veronika","family":"Pra\u017eienkov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ale\u0161","family":"Marek","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Malet\u00ednsk\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00543-2"},{"issue":"313","key":"e_1_2_2_3_1","first-page":"1","article-title":"CART in the regulation of appetite and energy homeostasis","volume":"8","author":"Lau J","year":"2014","journal-title":"Front Neurosci"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)88651-3"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2007.07.028"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2005.11.041"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.12.014"},{"key":"e_1_2_2_8_1","first-page":"288","article-title":"Conditions for succsesful labeling of oxidation sensitive peptides by NA [I\u2010125]\u2010IODO\u2010GEN (TM) system","author":"Elbert T","year":"2010","journal-title":"Journal of Labelled Compounds & Radiopharmaceuticals"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2007.07.022"}],"container-title":["Journal of Labelled Compounds and Radiopharmaceuticals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjlcr.3871","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jlcr.3871","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jlcr.3871","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jlcr.3871","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:27:01Z","timestamp":1760624821000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jlcr.3871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,10]]},"references-count":8,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["10.1002\/jlcr.3871"],"URL":"https:\/\/doi.org\/10.1002\/jlcr.3871","archive":["Portico"],"relation":{},"ISSN":["0362-4803","1099-1344"],"issn-type":[{"value":"0362-4803","type":"print"},{"value":"1099-1344","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,10]]},"assertion":[{"value":"2020-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}