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Purification of [<jats:sup>18<\/jats:sup>F]FLT, via solid phase extraction (SPE) after radiosynthesis, using a combination of different SPE cartridges, yielded satisfactory results, with radiochemical and chemical purity &gt;99%. While the non\u2010decay corrected radiochemical yield (RCY) with 20\u2009mg (24 \u03bcmole) of BOC\u2010Nosyl precursor was found to be 6.80\u2009\u00b1\u00a00.16%, the decay corrected radiochemical yield (RCY) was 9.95\u2009\u00b1\u00a00.24%. Residual acetone, acetonitrile, and ethanol levels were found to be 22.97\u2009\u00b1\u00a00.76, 109.08\u2009\u00b1\u00a00.93, and 7,666.45\u2009\u00b1\u00a03.7\u00a0ppm, respectively. A simplified method for solid\u2010phase purification of [<jats:sup>18<\/jats:sup>F]FLT was developed, circumventing the need for HPLC purification. Biodistribution in C57BL\/6 mice with B16F10 cell line\u2010induced melanoma showed tumor to blood ratio of ~3.8 at 90\u2009min. PET\/CT imaging of normal rabbit injected with [<jats:sup>18<\/jats:sup>F]FLT shows selective uptake in the bone marrow and small intestine. [<jats:sup>18<\/jats:sup>F]FLT was found to be excreted through the kidneys and get collected in the urinary bladder, 120\u2009min post injection. PET\/CT imaging performed in rabbit model at 30, 60, 90, and 120\u2009min post [<jats:sup>18<\/jats:sup>F]FLT injections showed concordance with tissue distribution kinetics of mice tumor model.<\/jats:p>","DOI":"10.1002\/jlcr.3974","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T03:13:42Z","timestamp":1650856422000},"page":"206-222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Automated radiochemical synthesis of pharmaceutical grade [<sup>18<\/sup>F]FLT using 3\u2010N\u2010Boc\u20105\u2032\u2010O\u2010dimethoxytrityl\u20103\u2032\u2010O\u2010nosyl\u2010thymidine precursor and its Sep\u2010Pak\u00ae purification employing selective elution from reversed phase"],"prefix":"10.1002","volume":"65","author":[{"given":"Arpit","family":"Mitra","sequence":"first","affiliation":[{"name":"Radiopharmaceuticals Laboratory Board of Radiation and Isotope Technology  Navi Mumbai 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