{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T08:09:35Z","timestamp":1789546175721,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":36,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Labelled Comp Radiopharmac"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We describe the synthesis and preclinical assessment of a novel technetium\u201099m\u2010labeled 5\u03b1\u2010reductase (5AR) inhibitor, a 5AR\u2010targeting drug, a target enzyme of prostate cancer (PCa) pathology, as a SPECT imaging probe. Finasteride was attached to 6\u2010hydrazinonicotinic acid (HYNIC) with HYNIC NHS ester chemistry. The HYNIC\u2013finasteride conjugate was identified by NMR, IR, MP analysis, high\u2010resolution ESI MS ([M\u2009+\u2009H]+\u2009m\/z 507.3190, \u0394ppm \u22123.9), and RP HPLC (Rt 13.9\u2009min, &gt;\u200998% purity).\n                    <jats:sup>99<\/jats:sup>\n                    \u1d50Tc radiolabeling using tricine\/EDDA co\u2010ligands resulted in &gt;\u200998% radiochemical purity. The radiotracer was highly stable in\u00a0vitro (&gt;\u200991% in PBS at 4\u2009h; \u2265\u200968% in serum at 16\u2009h) and had a logP value of \u22121.25\u2009\u00b1\u20090.08, with good hydrophilicity. Saturation binding assays in LNCaP cells had 5AR, with Kd and Bmax values of 3.10\u2009\u00b1\u20090.7\u2009nM and 82.44\u2009\u00b1\u20093.2 fmol\/10\n                    <jats:sup>6<\/jats:sup>\n                    cells. Biodistribution studies in LNCaP xenograft\u2010bearing mice showed high tumor uptake (5.62%\u2009\u00b1\u20090.32% ID\/g at 4\u2009h), which reduced in blocked groups (1.25%\u2009\u00b1\u20090.18% ID\/g). SPECT images offered selective tumor accumulation with good tumor\u2010to\u2010normal tissue contrast and renal clearance in tumor\u2010bearing rabbits. These findings suggest that\n                    <jats:sup>99<\/jats:sup>\n                    \u1d50Tc\u2010HYNIC\u2013finasteride is a promising SPECT radiotracer for noninvasive 5AR imaging in PCa.\n                  <\/jats:p>","DOI":"10.1002\/jlcr.4167","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T07:32:08Z","timestamp":1759822328000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Synthesis and Preclinical Evaluation of\n                    <sup>99<\/sup>\n                    \u1d50Tc\u2010HYNIC\u2013Finasteride: A Novel SPECT Radiotracer Targeting 5\u03b1\u2010Reductase in Prostate Cancer"],"prefix":"10.1002","volume":"68","author":[{"given":"Saba","family":"Shirin","sequence":"first","affiliation":[{"name":"Nuclear Medicine Research Laboratory, Institute of Chemical Sciences University of Peshawar  Peshawar Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3145-6070","authenticated-orcid":false,"given":"Syed\u00a0Qaiser","family":"Shah","sequence":"additional","affiliation":[{"name":"Nuclear Medicine Research Laboratory, Institute of Chemical Sciences University of Peshawar  Peshawar Khyber Pakhtunkhwa Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0905-481X","authenticated-orcid":false,"given":"Ralph","family":"Santos\u2010Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Nano\u2010Radiopharmacy, Nuclear Engineering Institute, Brazilian Nuclear Energy Commission  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derya","family":"Ilem\u2010Ozdemir","sequence":"additional","affiliation":[{"name":"Ege University Faculty of Pharmacy Department of Radiopharmacy  \u0130zmir 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