{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:48:37Z","timestamp":1787960917080,"version":"build-2784847793"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000938","name":"Robert E. Leet and Clara Guthrie Patterson Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000938","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020066","name":"Feldstein Medical Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA072720\u20105923"],"award-info":[{"award-number":["P30CA072720\u20105923"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIMD Reports"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glycerol kinase deficiency (GKD) is an X\u2010linked recessive disorder due to <jats:italic>glycerol kinase<\/jats:italic> (<jats:italic>GK<\/jats:italic>) gene mutations resulting in hyperglycerolermia, hyperglyceroluria, and \u201cpseudohypertriglyceridemia.\u201d In vivo glycerol metabolism has not been assessed in GKD. A 62\u2010year\u2010old man with suspected GKD and his extended family underwent whole exome sequencing and fasting blood work with two modes of lipid measurements: (1) standard lipase\u2010based methodology and (2) nuclear magnetic resonance (NMR). Two overnight fasted men with GKD and a heterozygote female carrier then underwent <jats:sup>13<\/jats:sup>C<jats:sub>3<\/jats:sub>\u2010glycerol infusion. Affected family members had a novel two\u2010nucleotide deletion in exon 5 of the <jats:italic>GK<\/jats:italic> gene (c.373_374del). Compared to their family members (<jats:italic>n<\/jats:italic>\u2009=\u200914), men with GKD (<jats:italic>n<\/jats:italic>\u2009=\u20095) had significantly lower total cholesterol levels (3.72\u2009\u00b1\u20090.70 vs. 4.77\u2009\u00b1\u20090.85\u2009mmol\/L, <jats:italic>p<\/jats:italic>\u2009=\u20090.024). Compared to NMR, lipase\u2010based assays overreported triglycerides (5.28\u2009\u00b1\u20091.38 vs. 0.81\u2009\u00b1\u20090.32, mmol\/L, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and underreported low\u2010density lipoprotein cholesterol values (0.93\u2009\u00b1\u20090.23 vs. 2.18\u2009\u00b1\u20090.42\u2009mmol\/L, <jats:italic>p<\/jats:italic>\u2009=\u20090.001) in GKD. Men with GKD could not convert glycerol into glucose or triglycerides, which was preserved in the heterozygote carrier. Glycolytic metabolism of glycerol to lactate persisted in GKD, but it was reduced by a magnitude and, possibly, due to homologous glycerol kinases encoded by other genes. In summary, we report a novel <jats:italic>GK<\/jats:italic> pathogenic variant; affected men cannot convert circulating glycerol to glucose or triglycerides and have lower cholesterol levels. These results offer a human model for potentially targeting glycerol kinase to treat conditions associated with hyperglycemia and hyperlipidemia and warrant further investigation.<\/jats:p>","DOI":"10.1002\/jmd2.12452","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T05:44:00Z","timestamp":1726638240000},"page":"392-400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In vivo glycerol metabolism in patients with glycerol kinase deficiency"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6701-3258","authenticated-orcid":false,"given":"Ankit","family":"Shah","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Metabolism and Nutrition, Department of Medicine, Robert Wood Johnson Medical School Rutgers University  New Brunswick New Jersey 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