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In this study we determined the nature of gut\u2010associated haemopoietic tissue in the goldfish, <jats:italic>Carassius auratus<\/jats:italic>, using light and electron microscopy. This tissue is a loose stroma of reticular cells and fibers vascularized by capillaries, venules, and arterioles. The cellular population includes lymphoblasts, small and medium\u2010sized lymphocytes, plasmocytes, macrophages, and various granulocytes. The most abundant granulocyte is the mast cell, whose large granules stain with Alcian blue and toluidine blue. Heterophils are found in the intestinal connective tissue as well as two other granulocytes: one with ovoid granules having dense parallel lamellae and another with granules containing crystalline inclusions. Immature forms of both granulocytes were also noted. Macrophages containing phagocytosed debris were often located close to the epithelium; they were observed forming clusters with lymphocytes. The epithelium contained a number of migrating leucocytes including lymphocytes and lymphoblasts, macrophages, and heterophils. Although many granulocytes were found in the connective tissue, granulopoiesis does not seem to be a major function. Gut\u2010associated haemopoietic tissue in goldfish resembles diffuse lymphoid tissue and may be involved in intestinal immune responses.<\/jats:p>","DOI":"10.1002\/jmor.1051900103","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T16:45:38Z","timestamp":1109263538000},"page":"9-26","source":"Crossref","is-referenced-by-count":36,"title":["Gut\u2010associated lymphoid tissue (GALT) of the goldfish, <i>Carassius auratus<\/i>"],"prefix":"10.1002","volume":"190","author":[{"given":"Robert J.","family":"Temkin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald B.","family":"McMillan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00213761"},{"key":"e_1_2_1_3_1","first-page":"84","article-title":"Studies on the lymphocytes in the intestinal epithelium of the chicken. 1. 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