{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:55:54Z","timestamp":1758819354204},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,6]],"date-time":"2005-02-06T00:00:00Z","timestamp":1107648000000},"content-version":"vor","delay-in-days":3630,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Morphology"],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Xenopus laevis<\/jats:italic> interphotoreceptor matrix (IPM) contains a relatively aqueous insoluble wheat germ agglutinin (WGA)\u2010binding component containing unidentifiied sialoglycoconjugates (Wood et al [1984] J. Comp. Neurol. <jats:italic>228<\/jats:italic>:299\u2013307). The appearance of WGA\u2010binding macromolecules in the IPM was assessed during late embryonic stages (32\u201345) and in retinal rudiment cultures, using lectin cyutochemistry and Western blotting techniques. Metabolic labeling of the neural retina versus retinal pigment epithelium (RPE)\u2010choroid of juvenile <jats:italic>Xenopus<\/jats:italic> with <jats:sup>35<\/jats:sup>S\u2010MET was also evaluated <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>. Lectin cytochemistry of eyes from developmental stages 32\u201342 demonstrated distinct WGA\u2010ferritin\u2010binding sites on the developing outer segment membranes and in the IPM compartment. At stages 44\u201346 extensive WGA\u2010binding domains were present as an extracellular network with other randomly scattered domains near the retinal pigment epithelium. Retinal rudiments from stage 32\u201333 were isolated and allowed to differentiate in hanging drop culture (Hollyfield and Witkowsky [1974] J. Exp. Zool. <jats:italic>189<\/jats:italic>:357\u2013377) with or without an iinvesting pigment epithelium. Cultures developing with RPE exhibited an elaborate IPM with an anastomosing meshwork of WGA\u2010ferritin binding sites. In the absence of RPE only limited amoutns of binding restricted to the immediate vicinity of the developing photoreceptor outer segement membranes was observed. When Western blots were probed with WGA\u2010HRP, stage 32\u201345 retinas demonstrated a major WGA\u2010binding band of 126 kD. Similar amounts of WGA\u2010binding macromolecules were synthesized in preparations cultured in the presence or absence of the investing RPE. During development the major WGA\u2010binding component is a 126\u2010kD protein. Equivalent synthesis of this protein in the presence and absence of RPE suggests that the PE is not required for synthesis of this 126\u2010kD component. These results suggest that the retina is the primary site of synthesis of the WGA\u2010binding components of the <jats:italic>Xenopus<\/jats:italic> IPM, whereas the PE plays a principal role in their assembly and organization. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmor.1052230308","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T14:15:59Z","timestamp":1109254559000},"page":"325-339","source":"Crossref","is-referenced-by-count":4,"title":["Development of the interphotoreceptor matrix in <i>Xenopus laevis<\/i>"],"prefix":"10.1002","volume":"223","author":[{"given":"Devjani","family":"Lahiri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Landers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joe G.","family":"Hollyfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(81)90025-7"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(82)90088-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4347(87)80009-9"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902700209"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(73)90224-8"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(73)90223-6"},{"key":"e_1_2_1_8_1","first-page":"447","article-title":"Observations on the rod and cone layer of the human retina: a light and electron microscopic study","volume":"2","author":"Fine B. S.","year":"1963","journal-title":"Invest. Ophthalmol."},{"key":"e_1_2_1_9_1","first-page":"97","volume-title":"The Structure of the Eye","author":"Frederick J.","year":"1982"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1242\/jcs.105.1.7","article-title":"Interphotoreceptor retinoid\u2010binding protein (IRBP), a major 124 kDa glycoprotein in the interphotoreceptor matrix of Xenopus laevis. Characterization, molecular cloning and biosynthesis","volume":"105","author":"Gonzales\u2010Fernandez F.","year":"1993","journal-title":"J. Cell Sci."},{"key":"e_1_2_1_11_1","first-page":"75","volume-title":"Methods in Developmental Biology","author":"Gurdon J. B.","year":"1967"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713688709025225"},{"key":"e_1_2_1_13_1","first-page":"207","volume-title":"Progress in Retinal Research","author":"Hageman G. S.","year":"1991"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-044276-8.50010-2"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(76)90040-3"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401890309"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901880406"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006982-198909010-00008"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(90)90177-V"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(90)90220-O"},{"key":"e_1_2_1_21_1","first-page":"531","volume-title":"Methods in Developmental Biology","author":"Jacobson A. G.","year":"1967"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"6633","DOI":"10.1016\/S0021-9258(19)69037-X","article-title":"The carbohydrate binding specificity of PEA and lentil lectins","volume":"256","author":"Kornfeld K.","year":"1981","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(92)90199-3"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051290405"},{"key":"e_1_2_1_25_1","volume-title":"Normal Table of Xenopus laevis (Daudin)","author":"Nieuwkoop P. D.","year":"1956"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(64)80084-8"},{"key":"e_1_2_1_27_1","first-page":"196","article-title":"Distribution and synthesis of sulfated mucopolyscaccharides in the retina of rat","volume":"5","author":"Ocumpaugh D. E.","year":"1966","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(70)80013-6"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(87)80101-X"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1958.tb39543.x"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(92)90194-W"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(86)80090-2"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(87)80135-5"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901010104"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902280214"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01675296"},{"key":"e_1_2_1_38_1","unstructured":"Zimmerman L. E.(1958) Applications of histochemical methods for the demonstration of acid mucopolysaccharides to ophthalmic pathology.Trans. Am. Acad. Ophthalmol.Sept.\/Oct:697\u2013703."},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)78054-7"}],"container-title":["Journal of Morphology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmor.1052230308","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmor.1052230308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T03:15:15Z","timestamp":1698376515000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmor.1052230308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,3]]}},"alternative-id":["10.1002\/jmor.1052230308"],"URL":"https:\/\/doi.org\/10.1002\/jmor.1052230308","archive":["Portico"],"relation":{},"ISSN":["0362-2525","1097-4687"],"issn-type":[{"value":"0362-2525","type":"print"},{"value":"1097-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,3]]}}}