{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:57:43Z","timestamp":1791982663876,"version":"4.4.0"},"reference-count":61,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,10,26]],"date-time":"2001-10-26T00:00:00Z","timestamp":1004054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Morphology"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To provide basic data about bone resorbing cells in the skeleton during the life cycle of <jats:italic>Danio rerio<\/jats:italic>, larvae, juveniles, and adults (divided into six age groups) were studied by histological procedures and by demonstration of the osteoclast marker enzyme tartrate\u2010resistant acid phosphatase (TRAP). Special attention was paid to the lower jaw, which is a standard element for fish bone studies. The presence of osteoclasts at endosteal surfaces of growing bones of all animals older than 20 days reveals that resorption is an important part of zebrafish skeletal development. The first bone\u2010resorbing cells to form are mononucleated. They appear in 20\u2010day\u2010old animals concurrently in the craniofacial skeleton and vertebral column. Mononucleated osteoclasts are predominant in juveniles. Regional differences characterize the appearance of osteoclasts; at thin skeletal elements (neural arches, nasal) mononucleated osteoclasts are predominant even in adults. Multinucleated bone\u2010resorbing cells were first observed in 40\u2010day\u2010old animals and are the predominant osteoclast type of adults. Both mono\u2010 and multinucleated osteoclasts contribute to allometric bone growth but multinucleated osteoclasts are also involved in lacunar bone resorption and repeated bone remodeling. Resorption of the dentary follows the pattern described above (mononucleated osteoclasts precede multinucleated cells) and includes the partial removal of Meckel's cartilage. Bone marrow spaces created by resorption are usually filled with adipose tissue. In conclusion, bone resorption is primarily subjected to the demands of growth, the appearance of mono\u2010 and multinucleated osteoclasts is site\u2010 and age\u2010related, and bone remodeling occurs. The results are discussed in relation to findings in other teleosts and in mammals. J. Morphol. 250:197\u2013207, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmor.1065","type":"journal-article","created":{"date-parts":[[2004,10,28]],"date-time":"2004-10-28T13:42:34Z","timestamp":1098970954000},"page":"197-207","source":"Crossref","is-referenced-by-count":167,"title":["Features of mono\u2010 and multinucleated bone resorbing cells of the zebrafish <i>Danio rerio<\/i> and their contribution to skeletal development, remodeling, and growth"],"prefix":"10.1002","volume":"250","author":[{"given":"P. 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