{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T11:07:25Z","timestamp":1769166445301,"version":"3.49.0"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Morphology"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The reproductive and adaptative behavior of wild rodents is synchronized primarily by the photoperiod. The viscacha, a South American rodent of nocturnal habits and seasonal reproduction is photoperiod\u2010dependent and its reproductive behavior is regulated by the retinohypothalamic\u2010pituitary pineal axis. Adult males exhibit an annual reproductive cycle with periods of maximum gonadal activity (summer\u2010early autumn) and gonadal regression (winter). The corpus and the cauda, the most sensitive segments of the epididymis to changes induced by the photoperiod, were analyzed using electron microscopy and enzymatic biochemistry. During gonadal regression, principal and clear cells showed signs of involution with respect to the activity period. These were characterized by more irregular nuclei, smaller cytoplasms, large vacuoles, altered mitochondria, and glycogen deposits. All cellular populations of the epididymal epithelium in regression presented abundant lysosome\u2010like dense bodies during the active period. In addition, we measured the activity of four acid glycosidases in the cauda epididymis along the reproductive cycle. N\u2010acetyl\u2010\u03b2\u2010D\u2010glucosaminidase (NAG), an enzyme that degrades endocytosed substances from the epididymal lumen, increased significantly during gonadal regression relative to the active period. These results demonstrate that the viscacha epididymis exhibits significant ultrastructural and biochemical changes during the reproductive cycle. We demonstrate that during regression, melatonin secretion in viscacha increases. This study shows that the epididymal epithelium is reduced. Thus, we postulate that the changes observed in the epididymis are modulated by pineal melatonin. Despite these changes, the epididymis might maintain a microenvironment suitable for the survival of stored spermatozoa. J. Morphol. 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmor.10718","type":"journal-article","created":{"date-parts":[[2009,1,3]],"date-time":"2009-01-03T07:33:16Z","timestamp":1230967996000},"page":"805-814","source":"Crossref","is-referenced-by-count":17,"title":["Ultrastructural and biochemical seasonal changes in epididymal corpus and cauda of viscacha (Lagostomus maximus maximus)"],"prefix":"10.1002","volume":"270","author":[{"given":"Claudia","family":"Aguilera\u2010Merlo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Fogal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tirso","family":"Sator","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Dominguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Sosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Scardapane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram\u00f3n","family":"Piezzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,6,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1996.tb01775.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.20144"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9930361"},{"key":"e_1_2_1_5_1","first-page":"7","article-title":"Ultrastructural study on the epithelium lining ducts epididymis in adult cats (Felis catus)","volume":"97","author":"Arrighi S","year":"1993","journal-title":"Arch Biol"},{"key":"e_1_2_1_6_1","first-page":"19","volume-title":"Lysosomes a Laboratory Handbook","author":"Barrett AJ","year":"1977"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/01485010252869289"},{"key":"e_1_2_1_8_1","unstructured":"Calder\u00f3nC.2001.Ritmos diarios y estacionales en retina y gl\u00e1ndula pineal delLagostomus maximus maximus. 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San Luis Argentina."},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.1997.19110077.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(19991201)256:4<335::AID-AR1>3.0.CO;2-I"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1993.tb01187.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yhbeh.2004.07.001"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-079X.1987.tb00858.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000212019"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.4081\/956"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000099626"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod45.3.493"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1993.1068"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/378922"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00457.2002"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001900404"},{"key":"e_1_2_1_22_1","first-page":"31","article-title":"Hidroxyindole\u2010o\u2010methyl transferase (HIOMT) and serotonin in the pineal gland of the viscacha (Lagostomus maximus maximus)","volume":"27","author":"Guzman J","year":"1977","journal-title":"Acta Physiol Latinoam"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01256636"},{"key":"e_1_2_1_24_1","first-page":"145","article-title":"Exposure to constant light, increase pineal weith and RNA content in the pineal gland of the viscacha (Lagostomus maximus maximus)","volume":"33","author":"Guzman J","year":"1983","journal-title":"Acta Physiol Latinoam"},{"key":"e_1_2_1_25_1","first-page":"2380","article-title":"Age\u2010related changes in the cat testis and epididymis","volume":"45","author":"Hart L","year":"1984","journal-title":"Am J Vet Res"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod36.3.709"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092110309"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001830202"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)62199-3"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2004.tb02760.x"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/43.5.7730593"},{"key":"e_1_2_1_32_1","first-page":"57","article-title":"Reproductive parameters of the plains viscacha (Lagostomus maximus maximus) in San Luis province, Argentina","volume":"2","author":"Jackson JE","year":"1989","journal-title":"Vida Silvestre Neotropical"},{"key":"e_1_2_1_33_1","first-page":"47","article-title":"A formaldhide\u2010glutaraldehyde fixative of high osmolarity for use in electron microscopy","volume":"11","author":"Karnovsky MJ","year":"1965","journal-title":"J Biophys Biochem Cytol"},{"key":"e_1_2_1_34_1","first-page":"289","article-title":"Ecolog\u00eda de la vizcacha (Lagostomus maximus maximus) en el nordeste de la provincia de Entre Rios","volume":"6","author":"Llanos AC","year":"1952","journal-title":"Rev Invest Agric Bs As"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1978.tb00511.x"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod1.Supplement_1.134"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2005.09.023"},{"key":"e_1_2_1_38_1","first-page":"23","article-title":"Seasonal variations and ultrastructure of colloid accumulation in the pituitary pars distalis of viscacha (Lagostomus maximus maximus)","volume":"18","author":"Mohamed F","year":"1994","journal-title":"Biocell"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(20000301)258:3<252::AID-AR4>3.0.CO;2-D"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-8166(97)80078-X"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199809)252:1<8::AID-AR2>3.0.CO;2-T"},{"key":"e_1_2_1_42_1","unstructured":"Mu\u00f1ozE.1998b.Estudio estacional del compartimiento tubular e intersticial del test\u00edculo de vizcacha (Lagostomus maximus maximus). Papel de la melatonina en la ciclicidad reproductiva y su relaci\u00f3n con el fotoper\u00edodo. Tesis Doctoral. Biblioteca central de la UNSL. 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