{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:38:49Z","timestamp":1791175129130,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2009,2,13]],"date-time":"2009-02-13T00:00:00Z","timestamp":1234483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Morphology"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Helicobacter pullorum<\/jats:italic> infections have been associated with several enterohepatic diseases, but the mechanism of action is currently undefined. The present study was therefore set up to investigate possible cytotoxic effects of this pathogen on liver cells. A mouse hepatic cell line was exposed to <jats:italic>H. pullorum<\/jats:italic> sonicate and cytotoxicity was observed for all isolates after incubation for 72 h. Features characteristic for mitotic catastrophe characterized by chromatin condensation, formation of multinuclear distended cells and micronucleation, were recorded. In addition, intranuclear pseudoinclusions were seen in sonicate\u2010treated cells. Finally, cells exposed to sonicate eventually underwent cell death with the morphological features of necrosis, which occurred without activation of caspase\u20103. The toxic factor involved in the cytotoxic activity proved to be soluble, trypsin\u2013sensitive and stable at 56\u00b0C and at \u221270\u00b0C with a molecular weight to be over 50 kDa. The current study shows for the first time that <jats:italic>H. pullorum<\/jats:italic> causes mitotic catastrophe resulting in primary necrosis in mouse hepatocytes. J. Morphol., 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmor.10730","type":"journal-article","created":{"date-parts":[[2009,2,13]],"date-time":"2009-02-13T20:12:44Z","timestamp":1234555964000},"page":"921-928","source":"Crossref","is-referenced-by-count":11,"title":["Mitotic catastrophe as a prestage to necrosis in mouse liver cells treated with <i>Helicobacter pullorum<\/i> sonicates"],"prefix":"10.1002","volume":"270","author":[{"given":"Liesbeth M.","family":"Ceelen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freddy","family":"Haesebrouck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"D'Herde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitri V.","family":"Krysko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman","family":"Favoreel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vandenabeele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Ducatelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemie","family":"Decostere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,2,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.1998.00438.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1613"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207528"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2005.04.002"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2984-2986.2005"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.10.020"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/44188"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00159-3"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400883"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/cbir.1999.0466"},{"key":"e_1_2_1_12_1","first-page":"124","article-title":"The expanding genus of Helicobacter: Pathogenic and zoonotic potential","volume":"8","author":"Fox JG","year":"1997","journal-title":"Semin Gastrointest Dis"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.5.1548-1558.1996"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70589-X"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409130102"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1083-4389.2004.00237.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/095530097143185"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401724"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2008.150"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2007.12.001"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(08)01416-X"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00174.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-4706hyp"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.1.2267"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-005-1002-7"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505009200"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.12.011"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i4.598"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20535"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"2070","DOI":"10.1128\/iai.64.6.2070-2078.1996","article-title":"Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter spp. cdtB genes","volume":"64","author":"Pickett CL","year":"1996","journal-title":"Infect Immun"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1054\/mehy.1999.0987"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1099\/0022-1317-49-5-473","article-title":"Characterisation of cytolethal distending toxin (CDT) of Campylobacter jejuni","volume":"49","author":"Purdy D","year":"2000","journal-title":"J Med Microbiol"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1054\/drup.2001.0213"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-007-0144-y"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-140-12-3441"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365549709019053"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-42-1-48"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10254-004-0030-8"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2008.47"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402286"},{"key":"e_1_2_1_41_1","article-title":"Study of Helicobacter pullorum proinflammatory properties on human epithelial cells in vitro","author":"Varon C","year":"2008","journal-title":"Gut"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-008-9131-1"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1375"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.1.184-191.2000"},{"key":"e_1_2_1_45_1","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1086\/315705","article-title":"Cytolethal distending toxin in avian and human isolates of H. pullorum","volume":"182","author":"Young VB","year":"2000","journal-title":"J Infect Dis"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2521-2527.2004"}],"container-title":["Journal of Morphology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmor.10730","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmor.10730","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T00:34:29Z","timestamp":1699922069000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmor.10730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,2,13]]},"references-count":45,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["10.1002\/jmor.10730"],"URL":"https:\/\/doi.org\/10.1002\/jmor.10730","archive":["Portico"],"relation":{},"ISSN":["0362-2525","1097-4687"],"issn-type":[{"value":"0362-2525","type":"print"},{"value":"1097-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,2,13]]}}}