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To better describe follicle assembly and seminiferous cord development, we used histochemical techniques to detect carbohydrate\u2010rich extracellular matrix components in 1\u2010week, 1\u2010month, 3\u2010month, and 5\u2010month\u2010old gonads. We found profound morphological changes in both ovary and testis. During this time, oogenesis progressed up to diplotene arrest and meiotic germ cells increasingly interacted with follicular cells. Concomitant with follicles becoming invested with full complements of granulosa cells, a periodic acid Schiff's (PAS)\u2010positive basement membrane formed. As follicles enlarged and thecal layers were observed, basement membranes and thecal compartments gained periodic acid\u2010methionine silver (PAMS)\u2010reactive fibers. The ovarian medulla increased first PAS\u2010 and then PAMS reactivity as it fragmented into wide lacunae lined with low cuboidal to squamous epithelia. During this same period, testicular germ cells found along the tubule margins were observed progressing from spermatogonia to round spermatids located within the center of tubules. Accompanying this meiotic development, interstitial Leydig cell clusters become more visible and testicular capsules thickened. During the observed testis development, the thickening tunica albuginea and widening interstitial tissues showed increasing PAS\u2010 and PAMS reactivity. We observed putative intersex structures in both ovary and testis. On the coelomic aspect of testes were cell clusters with germ cell morphology and at the posterior end of ovaries, we observed \u201cmedullary rests\u201d resembling immature testis cords. We hypothesize laboratory conditions accelerated gonad maturation due to optimum conditions, including nutrients and temperature. Laboratory alligators grew more rapidly and with increased body conditions compared with previous measured, field\u2010caught animals. Additionally, we predict the morphological maturation observed in these gonads is concomitant with increased endocrine activities. J. Morphol. 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmor.10818","type":"journal-article","created":{"date-parts":[[2009,12,9]],"date-time":"2009-12-09T22:20:50Z","timestamp":1260397250000},"page":"580-595","source":"Crossref","is-referenced-by-count":30,"title":["Posthatching development of <i>Alligator mississippiensis<\/i> ovary and testis"],"prefix":"10.1002","volume":"271","author":[{"given":"Brandon C.","family":"Moore","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather J.","family":"Hamlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole L.","family":"Botteri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley N.","family":"Lawler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ketan K.","family":"Mathavan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Louis J.","family":"Guillette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,12,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.2337\/diab.37.12.1695","article-title":"Phenotypic\u2010expression of collagen types in mesangial matrix of diabetic and nondiabetic rats","volume":"37","author":"Abrass CK","year":"1988","journal-title":"Diabetes"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00058.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2007.00726.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0742-8413(99)00100-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0742-8413(99)00110-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1987.tb00222.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00215155"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-006-0194-1"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-948555"},{"key":"e_1_2_1_11_1","unstructured":"CardeilhacPT.Reproductive physiology of the American alligator. 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