{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T19:28:15Z","timestamp":1775330895697,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T00:00:00Z","timestamp":1474329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005623","name":"North Carolina Sea Grant","doi-asserted-by":"publisher","award":["2014\u20101751"],"award-info":[{"award-number":["2014\u20101751"]}],"id":[{"id":"10.13039\/100005623","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Morphology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Observations of cuticular structures mineralized with silica within the Crustacea have been limited to the opal teeth of copepods, mandibles of amphipods, and recently the teeth of the gastric mill in the blue crab <jats:italic>Callinectes sapidus<\/jats:italic>. Copepod teeth are deposited during premolt, with sequential elaboration of organic materials followed by secretion of silica into the tooth mold. The timing of mineralization is in stark contrast to that of the general integument of crustaceans in which calcification is completely restricted to the postmolt period. To determine the timing of molt\u2010related deposition and silicification of the teeth of the gastric mill, the medial tooth of the blue crab <jats:italic>C. sapidus<\/jats:italic> was examined histologically and ultrastructurally across the molt cycle. Histological data revealed deposition of the organic matrix of the epicuticle and exocuticle during premolt. No evidence of postmolt changes in the thickness of the epicuticle and exocuticle, or any deposition of endocuticle, was observed. Scanning electron microscopy revealed degradation of the outer surface of the old tooth during premolt. During premolt, epithelial structures resembling papilla appeared to secrete a fibrous web that coalesces to become the matrix of the new tooth. Semi\u2010quantitative elemental analyses indicated simultaneous deposition of silica and organic matrix, and demonstrated a homogeneous distribution of silicon throughout the epicuticle of the tooth at all stages. However, there is evidence of deposition (presumably silicification) during postmolt as spaces between the papillae become filled in. Thus, the pattern and timing of deposition and silicification of the tooth are different from both teeth of copepods and the general exoskeleton of decapods, and may facilitate rapid resumption of feeding and consumption of the exuvia in early postmolt. J. Morphol. 277:1648\u20131660, 2016. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jmor.20614","type":"journal-article","created":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T03:32:48Z","timestamp":1474428768000},"page":"1648-1660","source":"Crossref","is-referenced-by-count":8,"title":["Silicification of the medial tooth in the blue crab <i>Callinectes sapidus<\/i>"],"prefix":"10.1002","volume":"277","author":[{"given":"Katherine T.","family":"Nesbit","sequence":"first","affiliation":[{"name":"Department of Biology and Marine Biology University of North Carolina Wilmington 601 S. College Road Wilmington North Carolina 28403"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert D.","family":"Roer","sequence":"additional","affiliation":[{"name":"Department of Biology and Marine Biology University of North Carolina Wilmington 601 S. College Road Wilmington North Carolina 28403"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,20]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"R484","article-title":"Kinetic analysis of electrogenic 2Na+\u22121H+ antiport in crustacean hepatopancreas","volume":"257","author":"Ahearn GA","year":"1989","journal-title":"Am J Physiol"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2307\/1549123"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1242\/jeb.119.1.275","article-title":"Post\u2010moult calcification in the blue crab (Callinectes sapidus): Relationships between apparent net H+ excretion, calcium and bicarbonate","volume":"119","author":"Cameron JN.","year":"1985","journal-title":"J Exp Biol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.2.361"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10311"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051630203"},{"key":"e_1_2_6_8_1","first-page":"103","article-title":"Mue et cycle d'intermue chez les Crustac\u00e9s D\u00e9capodes","volume":"19","author":"Drach P.","year":"1939","journal-title":"Ann Inst Oc\u00e9anogr Monaco"},{"key":"e_1_2_6_9_1","first-page":"595","article-title":"Sur la m\u00e9thode de d\u00e9termination des stades d'intermue et son application g\u00e9n\u00e9rale aux crustac\u00e9s","volume":"18","author":"Drach P","year":"1967","journal-title":"Vie Milieu"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4687(199906)240:3<267::AID-JMOR5>3.0.CO;2-8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-5944-2_17"},{"key":"e_1_2_6_12_1","first-page":"565","volume-title":"The Blue Crab, Callinectes sapidus","author":"Hines A.","year":"2007"},{"key":"e_1_2_6_13_1","first-page":"147","volume-title":"Microscopic Anatomy of Invertebrates","author":"Icely J","year":"1992"},{"key":"e_1_2_6_14_1","first-page":"440","volume-title":"Histology of the Blue Crab, Callinectes sapidus: A Model for the Decapoda","author":"Johnson PT.","year":"1980"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1434-4610(98)70029-X"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.2216\/i0031-8884-6-1-1.1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043073"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00465"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02114678"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.2307\/1541099"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/icv080"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/24.4.893"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.2307\/1548901"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-029X.99081"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127871"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20423"}],"container-title":["Journal of Morphology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmor.20614","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmor.20614","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmor.20614","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T20:30:34Z","timestamp":1696365034000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmor.20614"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,20]]},"references-count":25,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1002\/jmor.20614"],"URL":"https:\/\/doi.org\/10.1002\/jmor.20614","archive":["Portico"],"relation":{},"ISSN":["0362-2525","1097-4687"],"issn-type":[{"value":"0362-2525","type":"print"},{"value":"1097-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,20]]}}}