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They contain ovarian nests, individual follicles, inner germinal ovarian epithelium, and fat tissue. Nests comprise cystoblasts, germline cysts, numerous early previtellogenic oocytes, and somatic cells. Cysts are composed of cystocytes, which are connected by intercellular bridges and are in the pachytene stage of the first meiotic prophase. They contain bivalents, finely granular, medium electron dense material, and nucleoli in the nucleoplasm. Many cystocytes degenerate. Oocytes differ in size and structure. Most oocytes are in the pachytene and early diplotene stages and are referred to as the <jats:italic>PACH<\/jats:italic> oocytes. Oocytes in more advanced diplotene stage are referred to as the <jats:italic>DIP<\/jats:italic> oocytes. Nuclei in the <jats:italic>PACH<\/jats:italic> oocytes contain bivalents and irregularly shaped accumulation of DNA (DNA\u2010body), most probably corresponding to the rDNA\u2010body. The DNA\u2010body is composed of loose, fine granular material, and comprises multiple nucleoli. At peripheries, it is fragmented into blocks that remain in contact with the inner nuclear membrane. In the ooplasm, there is the rough endoplasmic reticulum, Golgi complexes, free ribosomes, complexes of mitochondria with cement, fine fibrillar material containing granules, and lipid droplets. The organelles and material of nuclear origin form a distinct accumulation (a granular ooplasm) in the vicinity of the nucleus. Some of the <jats:italic>PACH<\/jats:italic> oocytes are surrounded by flat somatic cells. There are lampbrush chromosomes and multiple nucleoli present (early diplotene stage) in the nucleoplasm. These <jats:italic>PACH<\/jats:italic> oocytes and neighboring somatic cells have initiated the formation of ovarian follicles. The remaining <jats:italic>PACH<\/jats:italic> oocytes transform to the <jats:italic>DIP<\/jats:italic> oocytes. The <jats:italic>DIP<\/jats:italic> oocytes contain lampbrush chromosomes and a DNA\u2010body is absent in nuclei. Multiple nucleoli are numerous in the nucleoplasm and granular ooplasm is present at the vegetal region of the oocyte.<\/jats:p>","DOI":"10.1002\/jmor.20723","type":"journal-article","created":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T06:56:41Z","timestamp":1499324201000},"page":"1438-1449","source":"Crossref","is-referenced-by-count":13,"title":["Ovarian nests in cultured females of the Siberian sturgeon <i>Acipenser baerii<\/i> (Chondrostei, Acipenseriformes)"],"prefix":"10.1002","volume":"278","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9199-3575","authenticated-orcid":false,"given":"Monika","family":"\u017belazowska","sequence":"first","affiliation":[{"name":"Department of Developmental Biology and Morphology of Invertebrates Institute of Zoology and Biomedical Research, Jagiellonian University in Krak\u00f3w, Gronostajowa 9 Krak\u00f3w 30\u2010387 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"Fopp\u2010Bayat","sequence":"additional","affiliation":[{"name":"Department of Ichthyology Faculty of Environmental Science, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5 Olsztyn 10\u2010917 Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,6]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"143","article-title":"Ovaries, oogenesis and insect phylogeny","volume":"36","author":"Bili\u0144ski S. 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