{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T03:55:40Z","timestamp":1769226940625,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T00:00:00Z","timestamp":1534464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Morphology"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although artificial insemination has been used for decades in <jats:italic>Penaeus monodon<\/jats:italic> aquaculture, the interaction of male and female external genitalia during spermatophore transfer has not been fully documented. As a result, studying the functional anatomy of this process may help to better refine the insemination technique. The sexual act in penaeoid prawns is virtually impossible to observe directly; as a result, this study aimed to describe the functional anatomy and interaction of external genitalia, such as the petasma, appendices masculinae and genital papillae of the male, with that of the thelycum and genital lobes of the female, using a combination of micro\u2010computed tomography and scanning electron microscopy. We hypothesise that the spermatophores are ejaculated into the ventromedial groove of the petasma and squeezed by abdominal flexure into the spermathecae of female's thelycum. During this process, the \u2018spike\u2010like\u2019 setae observed on the petasma and appendices masculinae are speculated to control the transferring direction of the spermatophores. The approach of three dimensional remodelling and animation reported in this study may prove useful in the examination of further hypotheses related to the functional anatomy of external genitalia and\/or appendages for other crustacean species.<\/jats:p>","DOI":"10.1002\/jmor.20875","type":"journal-article","created":{"date-parts":[[2018,8,17]],"date-time":"2018-08-17T10:40:59Z","timestamp":1534502459000},"page":"1346-1354","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The functional anatomy of external genitalia in the black tiger prawn (<scp><i>Penaeus monodon<\/i><\/scp>) studied by micro\u2010computed tomography and scanning electron microscopy"],"prefix":"10.1002","volume":"279","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6082-2466","authenticated-orcid":false,"given":"Tianyi","family":"Feng","sequence":"first","affiliation":[{"name":"School of Agriculture and Food Sciences University of Queensland  Gatton Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Paterson","sequence":"additional","affiliation":[{"name":"Queensland Department of Agriculture and Fisheries Bribie Island Research Centre  Woorim Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Panagiotopoulou","sequence":"additional","affiliation":[{"name":"Monash Biomedicine Discovery Institute School of Biomedical Sciences, Monash University  Clayton Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn","family":"Green","sequence":"additional","affiliation":[{"name":"Centre for Microscopy &amp; Microanalysis University of Queensland  St Lucia Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karine","family":"Mardon","sequence":"additional","affiliation":[{"name":"Centre for Advanced Imaging University of Queensland  St Lucia Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Johnston","sequence":"additional","affiliation":[{"name":"School of Agriculture and Food Sciences University of Queensland  Gatton Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,8,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2012.01.013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2013.06.023"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/00222937600770321"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2307\/1548173"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.7312\/baue90796-012","volume-title":"Crustacean Sexual Biology","author":"Bauer R. 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