{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:36:20Z","timestamp":1787589380218,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T00:00:00Z","timestamp":1681344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Morphology"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sperm transfer in spiders is achieved by copulatory organs on the male pedipalps (i.e., copulatory bulbs), which can be simple or a complex set of sclerites and membranes. During copulation, these sclerites can be used to anchor in corresponding structures in the female genitalia by means of hydraulic pressure. In the most diverse group of Entelegynae spiders, the retrolateral tibial apophysis clade, the female role in the coupling of genitalia is considered rather passive, as conformational changes of the female genital plate (i.e., the epigyne) during copulation are scarce. Here, we reconstruct the genital mechanics of two closely related species belonging to the\n                    <jats:italic>Aysha prospera<\/jats:italic>\n                    group (Anyphaenidae) that bear a membranous, wrinkled epigyne and male pedipalps with complex tibial structures. By using microcomputed tomography data\u00a0of a cryofixed mating pair, we reveal that most of the epigyne remains greatly inflated during genital coupling, and that the male tibial structures are coupled to the epigyne by the inflation of a tibial hematodocha. We propose that a turgent female vulva is a prerequisite for the genital coupling, which could implicate a female control device, and that the structures from the male copulatory bulb have been functionally replaced by tibial structures in these species. Furthermore, we show that the conspicuous median apophysis is maintained in spite of being functionally redundant, posing a puzzling situation.\n                  <\/jats:p>","DOI":"10.1002\/jmor.21586","type":"journal-article","created":{"date-parts":[[2023,4,13]],"date-time":"2023-04-13T03:25:00Z","timestamp":1681356300000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["MicroCT analysis unveils the role of inflatable female genitalia and male tibial complex in the genital coupling in the spider genus\n                    <i>Aysha<\/i>\n                    (Anyphaenidae, Araneae)"],"prefix":"10.1002","volume":"284","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7108-1379","authenticated-orcid":false,"given":"Dante","family":"Poy","sequence":"first","affiliation":[{"name":"Division of Arachnology Museo Argentino de Ciencias Naturales\u2014CONICET Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Norberto","family":"Piacentini","sequence":"additional","affiliation":[{"name":"Division of Arachnology Museo Argentino de Ciencias Naturales\u2014CONICET Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Michalik","sequence":"additional","affiliation":[{"name":"Zoologisches Institut und Museum Universit\u00e4t Greifswald  Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shou\u2010Wang","family":"Lin","sequence":"additional","affiliation":[{"name":"Zoologisches Institut und Museum Universit\u00e4t Greifswald  Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00edn Javier","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Division of Arachnology Museo Argentino de Ciencias Naturales\u2014CONICET Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,4,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1978.tb03336.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0073-47212000000200001"},{"key":"e_1_2_10_4_1","first-page":"23","article-title":"Revis\u00e3o do grupo prospera do g\u00eanero Aysha Keyserling, 1891 na regi\u00e3o Neotropical (Araneae, Anyphaenidae)","volume":"72","author":"Brescovit A. D.","year":"1992","journal-title":"Iheringia, S\u00e9rie Zoologia"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0101-81751996000500001"},{"key":"e_1_2_10_6_1","first-page":"230","volume-title":"The comity of spiders","author":"Bristowe W. S.","year":"1941"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.5479\/si.00810282.422"},{"key":"e_1_2_10_8_1","first-page":"249","volume-title":"The evolution of primary sexual characters in animals","author":"Eberhard W. G.","year":"2010"},{"key":"e_1_2_10_9_1","volume-title":"Biology of spiders","author":"Foelix R.","year":"2011"},{"issue":"4","key":"e_1_2_10_10_1","first-page":"1","article-title":"Structure and function of the genitalia in some American agelenid spiders","volume":"121","author":"Gering R. 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J.","year":"1969","journal-title":"Zoologische Verhandelingen"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-6395.1994.tb00974.x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.1995.tb00931.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1995.tb01778.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10255"},{"issue":"1","key":"e_1_2_10_20_1","first-page":"95","article-title":"A revision of the Afrotropical tracheline sac spider genus Thysanina Simon, 1910 (Araneae: Corinnidae)","volume":"47","author":"Lyle R.","year":"2006","journal-title":"African Invertebrates"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.11646\/zootaxa.4914.1.1"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2015.09.074"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/eth.13136"},{"key":"e_1_2_10_24_1","first-page":"326","article-title":"Beitr\u00e4ge zur Kenntnis des Kopulationsapparates einiger Spinnen","volume":"119","author":"Osterloh A.","year":"1922","journal-title":"Zeitschrift f\u00fcr Wissenschaftliche Zoologie"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.21095"},{"key":"e_1_2_10_26_1","first-page":"87","article-title":"Mechanics of copulation in Lycosa chaperi Simon (Araneida: Lycosidae)","volume":"2","author":"Sadana G. L.","year":"1972","journal-title":"Bulletin of the British Arachnological"},{"key":"e_1_2_10_27_1","first-page":"333","article-title":"Mechanics of copulation in Lycosa wroughtoni Pocock (Lycosidae: Araneida)","volume":"28","author":"Sadana G. L.","year":"1981","journal-title":"Zoologica Poloniae"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1096-3642.1997.tb01281.x"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"issue":"1","key":"e_1_2_10_30_1","first-page":"87","article-title":"Copulatory mechanisms in Zelotes, Drassyllus and Trachyzelotes (Araneae, Gnaphosidae), with additional faunistic and taxonomic data on species from southwest Europe","volume":"77","author":"Senglet A.","year":"2004","journal-title":"Mitteilungen der Schweizerischen entomologischen Gesellschaft"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1163\/156854297X00274"},{"key":"e_1_2_10_32_1","first-page":"145","article-title":"Female genital morphology and sperm priority patterns in spiders (Araneae)","volume":"2000","author":"Uhl G.","year":"2000","journal-title":"European Arachnology"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2007.07.003"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1664\/13-RA-001.1"}],"container-title":["Journal of Morphology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmor.21586","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T23:32:26Z","timestamp":1692660746000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmor.21586"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,13]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1002\/jmor.21586"],"URL":"https:\/\/doi.org\/10.1002\/jmor.21586","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/JMOR.21586\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JMOR.21586\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JMOR.21586\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JMOR.21586\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/JMOR.21586\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0362-2525","1097-4687"],"issn-type":[{"value":"0362-2525","type":"print"},{"value":"1097-4687","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,13]]},"assertion":[{"value":"2023-02-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e21586"}}