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Here, we present a drying method for SEM imaging of late frog embryos using commonly available dehydration solvents such as ethanol or methanol, xylene, and applying low temperature vacuum freeze drying. Briefly, embryos from early embryonic gills development to hatching were fixed with a paraformaldehyde\u2014glutaraldehyde mix, then dehydrated to ethanol or methanol, and then slowly dried using low temperature and constant vacuum pressure. An extra step of clearing using xylene after ethanol dehydration improved results considerably. 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