{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T11:29:57Z","timestamp":1771241397235,"version":"3.50.1"},"reference-count":63,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T00:00:00Z","timestamp":1755302400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Morphology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The white\u2010eared opossum,\n                    <jats:italic>Didelphis albiventris<\/jats:italic>\n                    , is an opportunistic and omnivorous marsupial, whose diet ranges from wild fruits to eggs and birds. Salivary glycoproteins play a key role in the protection of the oral cavity and the formation of the food bolus. Despite the importance of salivary glycoproteins, their detailed investigation in the white\u2010eared is lacking. This study investigated the morphology and glycan composition of the mandibular salivary glands of the white\u2010eared opossum for the first time. Histological and histochemical investigations were conducted on tissue fragments fixed with 4% PBS\u2010buffered paraformaldehyde and embedded in Paraplast. The pattern of glycoproteins was investigated using traditional histochemical methods (PAS, Alcian Blue pH 2.5, and High\u2010Iron Diamine staining) and lectin histochemistry. The glandular parenchyma consisted of acinar secretory units and a duct system characterized by abundant striated ducts. Secretory acini secrete neutral glycans and non\u2010sulfated acid glycans. Mannosylated N\u2010linked glycans terminating in \u03b12,6\u2010sialic acid and fucose are expressed in the secretory acini, containing intraluminal \u03b12,3\u2010sialylated O\u2010linked glycans. The epithelial lining of the striated and interlobular ducts also shows O\u2010linked glycans with terminal Gal\u03b21, 3GalNAc, and\n                    <jats:italic>\u03b1<\/jats:italic>\n                    GalNAc residues. Finally, the epithelium and lumen of interlobular ducts are enriched with additional GalNAc\u2010terminated O\u2010linked glycans with the appearance of lactosaminated glycans and the disappearance of \u03b12,3\u2010sialylated glycans. These results suggest that the saliva produced by the mandibular gland of the white\u2010eared opossum consists of a species\u2010specific pattern of glycoproteins, to whose composition the ductal system also contributes. The observed glycan composition is probably related to the diet of the white\u2010eared opossum and its adaptations to the environment and food availability. These results indicate that the mandibular salivary gland of the white\u2010eared opossum\n                    <jats:italic>Didelphis albiventris<\/jats:italic>\n                    has specific histological and molecular characteristics compared to other marsupial species, suggesting that diet and habitat, but not the taxonomic group, influence the mandibular gland features.\n                  <\/jats:p>","DOI":"10.1002\/jmor.70074","type":"journal-article","created":{"date-parts":[[2025,8,16]],"date-time":"2025-08-16T07:49:50Z","timestamp":1755330590000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Morphology and Glycan Composition of the Mandibular Glands in the White\u2010Eared Opossum (\n                    <i>Didelphis albiventris<\/i>\n                    )"],"prefix":"10.1002","volume":"286","author":[{"given":"Bruno Cesar","family":"Schimming","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, Graduate Program in Wild Animals, Botucatu S\u00e3o Paulo State University (UNESP) S\u00e3o Paulo Brazil"},{"name":"Institute of Biosciences of Botucatu, Laboratory of Wildlife Anatomy Botucatu, S\u00e3o Paulo State University (UNESP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline Herrera","family":"Farha","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, Graduate Program in Wild Animals, Botucatu S\u00e3o Paulo State University (UNESP) S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tais Harumi de Castro","family":"Sasahara","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, Graduate Program in Wild Animals, Botucatu S\u00e3o Paulo State University (UNESP) S\u00e3o Paulo Brazil"},{"name":"Institute of Biosciences of Botucatu, Laboratory of Wildlife Anatomy Botucatu, 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