{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T10:14:06Z","timestamp":1746612846643},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,11,25]],"date-time":"2009-11-25T00:00:00Z","timestamp":1259107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Staphylococcal enterotoxin B (SEB) is an exotoxin produced by <jats:italic>Staphylococcus aureus<\/jats:italic> and commonly associated with food poisoning. In this study, SEB\u2010binding peptides were identified by screening a phage displayed peptide library. The binding of peptides to SEB was tested with isothermal titration calorimetry (ITC) and of the five selected peptides, three showed affinity to SEB, with one measured to have the highest affinity constant (10<jats:sup>5<\/jats:sup>\u2009M<jats:sup>\u22121<\/jats:sup>). ITC revealed that the interaction of peptide ligands with SEB was driven entropically and the binding was dominated by hydrophobic interactions. Circular dichroism (CD) measurements and molecular dynamics (MD) simulations, together, give a structural insight into the interaction of peptides with SEB. While SEB binding peptides showed random coil structure before binding, after complex formation they had more ordered structures. The peptide with highest affinity to SEB showed stable conformation during MD simulation. Taken together, our approach about thermodynamic and structural characterization of peptide ligands can be used to develop aptamers, with high affinity and selectivity, for biosensor applications. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jmr.1003","type":"journal-article","created":{"date-parts":[[2009,11,25]],"date-time":"2009-11-25T21:20:52Z","timestamp":1259184052000},"page":"369-378","source":"Crossref","is-referenced-by-count":8,"title":["Thermodynamic and structural analysis of interactions between peptide ligands and SEB"],"prefix":"10.1002","volume":"23","author":[{"given":"Fahriye Ceyda","family":"Dudak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esra Acar","family":"Soykut","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat Erman","family":"O\u011fuz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatih","family":"Ya\u015far","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130smail Hakk\u0131","family":"Boyac\u0131","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,6,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.448118"},{"key":"e_1_2_6_3_1","first-page":"331","volume-title":"Interaction Models for Water in Relation to Protein Hydration","author":"Berendsen HJC","year":"1981"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.2408420"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.464397"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/14.10.747"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.01.020"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.470117"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099-1352(200011\/12)13:6<382::AID-JMR511>3.0.CO;2-W"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2008.02.017"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00838a031"},{"key":"e_1_2_6_12_1","first-page":"661","article-title":"Detection of staphylococcal enterotoxin B employing a piezoelectric crystal immunosensor","volume":"7","author":"Harteveld JLN","year":"1997","journal-title":"J. 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