{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T01:36:22Z","timestamp":1788399382393,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,1,25]],"date-time":"2010-01-25T00:00:00Z","timestamp":1264377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polyaniline\/carbon nanotubes composite (PANI\u2010CNT) electrochemically deposited onto indium\u2010tin\u2010oxide (ITO) coated glass plate has been utilized for <jats:italic>Neisseria gonorrhoeae<\/jats:italic> detection by immobilizing 5\u2032\u2010amino\u2010labeled <jats:italic>Neisseria gonorrhoeae<\/jats:italic> probe (aDNA) using glutaraldehyde as a cross\u2010linker. PANI\u2010CNT\/ITO and aDNA\u2010Glu\u2010PANI\u2010CNT\/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FT\u2010IR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect <jats:italic>N. gonorrhoeae<\/jats:italic> using methylene blue (MB) as redox indicator with response time of 60\u2009s and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>\u2009M to 1\u2009\u00d7\u200910<jats:sup>\u221217<\/jats:sup>\u2009M with detection limit of 1.2\u2009\u00d7\u200910<jats:sup>\u221217<\/jats:sup>\u2009M. Further, this bioelectrode (aDNA\u2010Glu\u2010PANI\u2010CNT\/ITO) exhibits specificity toward <jats:italic>N. gonorrhoeae<\/jats:italic> species and shows negative response with non\u2010<jats:italic>Neisseria gonorrhoeae<\/jats:italic> <jats:italic>Neisseria<\/jats:italic> species (NgNS) and other gram negative bacteria (GNB). 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