{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T03:16:22Z","timestamp":1788059782682,"version":"build-2784847793"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,7,8]],"date-time":"2013-07-08T00:00:00Z","timestamp":1373241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>Knowledge of drug\u2013target interaction is critical to our understanding of drug action and can help design better drugs. Due to the lack of adequate single\u2010molecule techniques, the information of individual interactions between ligand\u2010receptors is scarce until the advent of atomic force microscopy (AFM) that can be used to directly measure the individual ligand\u2010receptor forces under near\u2010physiological conditions by linking ligands onto the surface of the AFM tip and then obtaining force curves on cells. Most of the current AFM single\u2010molecule force spectroscopy experiments were performed on cells grown <jats:italic>in vitro<\/jats:italic> (cell lines) that are quite different from the human cells <jats:italic>in vivo<\/jats:italic>. From the view of clinical practice, investigating the drug\u2013target interactions directly on the patient cancer cells will bring more valuable knowledge that may potentially serve as an important parameter in personalized treatment. Here, we demonstrate the capability of AFM to measure the binding force between target (CD20) and drug (rituximab, an anti\u2010CD20 monoclonal antibody targeted drug) directly on lymphoma patient cancer cells under the assistance of ROR1 fluorescence recognition. ROR1 is a receptor expressed on some B\u2010cell lymphomas but not on normal cells. First, B\u2010cell lymphoma Raji cells (a cell line) were used for ROR1 fluorescence labeling and subsequent measurement of CD20\u2010rituximab binding force. The results showed that Raji cells expressed ROR1, and the labeling of ROR1 did not influence the measurement of CD20\u2010rituximab binding force. Then the established experimental procedures were performed on the pathological samples prepared from the bone marrow of a follicular lymphoma patient. Cancer cells were recognized by ROR1 fluorescence. Under the guidance of fluorescence, with the use of a rituximab\u2010conjugated tip, the cellular topography was visualized by using AFM imaging and the CD20\u2010Rituximab binding force was measured by single\u2010molecule force spectroscopy. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jmr.2287","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T02:07:16Z","timestamp":1373335636000},"page":"432-438","source":"Crossref","is-referenced-by-count":37,"title":["Atomic force microscopy study of the antigen\u2010antibody binding force on patient cancer cells based on ROR1 fluorescence recognition"],"prefix":"10.1002","volume":"26","author":[{"given":"Mi","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences  Shenyang 110016 China"},{"name":"University of Chinese Academy of Sciences  Beijing 100049 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiubin","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences  Beijing 100071 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