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In this work, the interactions of RNAs reproducing bacterial and human ribosomal A\u2010site, with two well\u2010known antibiotic aminoglycosides, Paromomycin and Neomycin, as well as several Neomycin\u2010dinucleotide and \u2010diPNA conjugates, have been evaluated by ITC and the corresponding thermodynamic quantities determined. The comparison of the thermodynamic data of aminoglycosides and their chemical analogues allowed to select Neomycin\u2010diPNA conjugates as the best candidates for antimicrobial activity. 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