{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T05:46:16Z","timestamp":1782539176616,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,4,19]],"date-time":"2016-04-19T00:00:00Z","timestamp":1461024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Iranian National Sciences Foundation (INSF)"},{"name":"Applied Microbiology Research Center (AMRC) of Baqiyatallah University of Medical Sciences (BUMS)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p><jats:italic>Staphylococcus aureus<\/jats:italic> are potent human pathogens possessing arsenal of virulence factors. Staphylococcal food poisoning (SFP) and respiratory infections mediated by staphylococcal enterotoxin B (SEB) are common clinical manifestations. Many diagnostic techniques are based on serological detection and quantification of SEB in different food and clinical samples. Aptamers are known as new therapeutic and detection tools which are available in different ssDNA, dsDNA and protein structures. In this study, we used a new set of ssDNA aptamers against SEB. The methods used included preparation of a dsDNA library using standard SEB protein as the target analyte, affinity chromatography matrix in microfuge tubes, SELEX procedures to isolate specific ssDNA\u2010aptamer as an affinity ligand, aptamer purification using ethanol precipitation method, affinity binding assay using ELISA, aptamer cloning and specificity test. Among 12 readable sequences, three of them were selected as the most appropriate aptamer because of their affinity and specificity to SEB. This study presents a new set of ssDNA aptamer with favorable selectivity to SEB through 12 rounds of SELEX. Selected aptamers were used to detect SEB in infected serum samples. Results showed that SEB c1 aptamer (2\u2009\u00b5g SEB\/100\u2009nM aptamer) had favorable specificity to SEB (<jats:italic>k<jats:sub>d<\/jats:sub><\/jats:italic>\u2009=\u20092.3\u2009\u00d7\u200910<jats:sup>\u221211<\/jats:sup>). In conclusion, aptamers can be considered as useful tools for detecting and evaluating SEB. The results showed that affinity chromatography was an affordable assay with acceptable accuracy to isolate sensitive and selective novel aptamers. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jmr.2542","type":"journal-article","created":{"date-parts":[[2016,4,19]],"date-time":"2016-04-19T07:54:06Z","timestamp":1461052446000},"page":"436-445","source":"Crossref","is-referenced-by-count":33,"title":["Isolation of a new ssDNA aptamer against staphylococcal enterotoxin B based on CNBr\u2010activated sepharose\u20104B affinity chromatography"],"prefix":"10.1002","volume":"29","author":[{"given":"Mojtaba","family":"Hedayati Ch","sequence":"first","affiliation":[{"name":"Applied Microbiology Research Center Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jafar","family":"Amani","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Sedighian","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Amin","sequence":"additional","affiliation":[{"name":"Department of Drug and Food Control, and Pharmaceutical Quality Assurance Research Center, Faculty of Pharmacy Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jafar","family":"Salimian","sequence":"additional","affiliation":[{"name":"Chemical Injuries Research Center Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raheleh","family":"Halabian","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas Ali","family":"Imani Fooladi","sequence":"additional","affiliation":[{"name":"Applied Microbiology Research Center Baqiyatallah University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins2071751"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(87)90187-6"},{"issue":"1","key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.2144\/02321dd04","article-title":"Research report use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods","volume":"32","author":"Bruno JG","year":"2002","journal-title":"Biotechniques"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.105528"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2012.11.021"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033410"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3141"},{"issue":"11","key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"5209","DOI":"10.4049\/jimmunol.160.11.5209","article-title":"Cutting edge: novel RNA ligands able to bind CD4 antigen and inhibit CD4+ T lymphocyte function","volume":"160","author":"Kraus E","year":"1998","journal-title":"J. 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