{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:28:04Z","timestamp":1789147684641,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T00:00:00Z","timestamp":1470268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","award":["01(2769)\/13\/EMR\u2010II"],"award-info":[{"award-number":["01(2769)\/13\/EMR\u2010II"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Neisseria meningitidis,<\/jats:italic> a human\u2010specific bacterial pathogen causes bacterial meningitis by invading the meninges (outer lining) of central nervous system. It is the polysaccharide present on the bacterial capsid that distinguishes various serogroups of <jats:italic>N.\u00a0meningitidis<\/jats:italic> and can be utilized as antigens to elicit immune response. A computational approach identified candidate T\u2010cell epitopes from outer membrane proteins Por B of <jats:italic>N.\u00a0meningitidis<\/jats:italic> (MC58): (<jats:sup>273<\/jats:sup>KGLVDDADI<jats:sup>282<\/jats:sup> in loop VII and <jats:sup>170<\/jats:sup>GRHNSESYH<jats:sup>179<\/jats:sup> in loop IV) present on the exposed surface of immunogenic loops of class 3 outer membrane proteins allele of <jats:italic>N.\u00a0meningitidis<\/jats:italic>. One of them, KGLVDDADI is used here for designing a diagnostic tool via molecularly imprinted piezoelectric sensor (molecularly imprinted polymer\u2010quartz crystal microbalance) for <jats:italic>N.\u00a0meningitidis<\/jats:italic> strain MC58. Methacrylic acid, ethylene glycol dimethacrylate and azoisobutyronitrile were used as functional monomer, cross\u2010linker and initiator, respectively. The epitope can be simultaneously bound to methacrylic acid and fitted into the shape\u2010selective cavities. On extraction of epitope sequence from thus grafted polymeric film, shape\u2010selective and sensitive sites were generated on electrochemical quartz crystal microbalance crystal, ie, known as epitope imprinted polymers. Imprinting was characterized by atomic force microscopy images. The epitope\u2010imprinted sensor was able to selectively bind <jats:italic>N.\u00a0meningitidis<\/jats:italic> proteins present in blood serum of patients suffering from brain fever. Thus, fabricated sensor can be used as a diagnostic tool for meningitis disease.<\/jats:p>","DOI":"10.1002\/jmr.2557","type":"journal-article","created":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T07:43:40Z","timestamp":1470296620000},"page":"572-579","source":"Crossref","is-referenced-by-count":31,"title":["An epitope\u2010imprinted piezoelectric diagnostic tool for <i>Neisseria meningitidis<\/i> detection"],"prefix":"10.1002","volume":"29","author":[{"given":"Neha","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of Chemistry, MMV Banaras Hindu University  Varanasi U.P. \u2010221005 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavita","family":"Shah","sequence":"additional","affiliation":[{"name":"Institute of Environment and Sustainable Development Banaras Hindu University  Varanasi U.P. \u2010221005 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenakshi","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, MMV Banaras Hindu University  Varanasi U.P. \u2010221005 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,4]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"53","article-title":"Structural simulation of MHC\u2010peptide interactions using T\u2010cell epitope in iron\u2010acquisition protein of N.\u00a0meningitidis for vaccine design","volume":"1","author":"Mishra N","year":"2010","journal-title":"Journal of Proteins and Proteomics"},{"key":"e_1_2_6_3_1","first-page":"351","article-title":"Bioinformatics approach for screening and modelling of putative T cell epitopes from Por B protein of Neisseria meningitidis as vaccine constructs","volume":"9","author":"Shah K","year":"2010","journal-title":"Indian J Biotechnol"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2007.12.004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2007.12.006"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1517\/14622416.5.6.597"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00407-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.6026\/97320630006255"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.019"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1212"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.10.5611"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.88.7.601"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(03)00623-9"},{"key":"e_1_2_6_14_1","first-page":"115","volume-title":"Meningococcal disease","author":"Cartwright K","year":"1995"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.Suppl.S134"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.56.6.417"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)61187-8"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","unstructured":"FeaversIM MariagraziaP2013.Meningococcal protein antigens and vaccines. 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