{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:53:14Z","timestamp":1789779194674,"version":"4.0.1"},"reference-count":93,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T00:00:00Z","timestamp":1477612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["NG 133\/1\u20101"],"award-info":[{"award-number":["NG 133\/1\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heparin\u2010induced thrombocytopenia (HIT), occurring up to approximately 1% to 5% of patients receiving the antithrombotic drug heparins, has a complex pathogenesis involving multiple partners ranging from small molecules to cells\/platelets. Recently, insights into the mechanism of HIT have been achieved by using single\u2010molecule force spectroscopy (SMFS), a methodology that allows direct measurements of interactions among HIT partners. Here, the potential of SMFS in unraveling the mechanism of the initial steps in the pathogenesis of HIT at single\u2010molecule resolution is highlighted. The new findings ranging from the molecular binding strengths and kinetics to the determination of the boundary between risk and non\u2010risk heparin drugs or platelet\u2010surface and platelet\u2010platelet interactions will be reviewed. These novel results together have contributed to elucidate the mechanisms underlying HIT and demonstrate how SMFS can be applied to develop safer drugs with a reduced risk profile.<\/jats:p>","DOI":"10.1002\/jmr.2585","type":"journal-article","created":{"date-parts":[[2016,10,28]],"date-time":"2016-10-28T07:43:28Z","timestamp":1477640608000},"source":"Crossref","is-referenced-by-count":16,"title":["Single\u2010molecule force spectroscopy applied to heparin\u2010induced thrombocytopenia"],"prefix":"10.1002","volume":"30","author":[{"given":"Thi\u2010Huong","family":"Nguyen","sequence":"first","affiliation":[{"name":"Institute for Immunology and Transfusion Medicine, University Medicine Greifswald  17475 Greifswald Germany"},{"name":"ZIK HIKE \u2010 Center for Innovation Competence, Humoral Immune Reactions in Cardiovascular Diseases University of Greifswald  17489 Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/14.2.121"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1411910"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0000000000000259"},{"key":"e_1_2_7_5_1","unstructured":"Michelson A.D.Platelets.Academic Press\/Elsevier;2013"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH13-08-0634"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-559518"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-12-027698"},{"key":"e_1_2_7_9_1","first-page":"265","article-title":"The hemostatic plug","volume":"14","author":"Sixma JJ","year":"1977","journal-title":"Semin Hematol"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-06-359836"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200300640"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwh051"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.me.40.020189.000335"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2009.08.017"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2nr32821f"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4557-2296-9.00025-7"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja045613g"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja057584v"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja038244h"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja073098r"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1459"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-04-1544"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2011.12.002"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-04-1546"},{"key":"e_1_2_7_25_1","first-page":"911","article-title":"Heparin\u2010induced thrombocytopenia is more common with unfractionated heparin than with low\u2010molecular\u2010weight heparin","volume":"69","author":"Warkentin TE","year":"1993","journal-title":"Thromb Haemost"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc070346"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5NR02132D"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1074"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(94)80963-4"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40169-015-0050-9"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAT.0b013e31815d6898"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-6576.2009.01947.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199609)32:1<65::AID-JBM8>3.0.CO;2-N"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2014.09.040"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat2903"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-013-9825-1"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2004.02.010"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3991(99)00135-7"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1994.34594249047.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V67.1.27.27"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24006"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1039\/B811426A"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth871"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1218"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6107(00)00014-6"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.1081"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.181"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.77.043007.090225"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.481598"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-007-0413-1"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2009.007"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-4484\/21\/7\/075101"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35559"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35174"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1037"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2004.04.029"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja1092002"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.11.011"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn505135d"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.104901"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.108.138842"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn1009648"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2013.01.006"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(02)73987-8"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1021\/la403237f"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.066"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/35014000"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3sm52609g"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000238350.89477.88"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.042879"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(03)74692-X"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03245784"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.202"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(97)78802-7"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1202946109"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2013.03.014"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn400705u"},{"key":"e_1_2_7_78_1","first-page":"1","article-title":"Rupture forces among human blood platelets at different degrees of activation","volume":"6","author":"Nguyen TH","year":"2016","journal-title":"Sci Rep\u2010Uk."},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0126624"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1067\/mlc.2002.127715"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.99.6.2140"},{"key":"e_1_2_7_82_1","first-page":"22","article-title":"Integrin and glycocalyx mediated contributions to cell adhesion identified by single cell force spectroscopy","author":"Boettiger D","year":"2010","journal-title":"J Phys\u2010Condens Mat."},{"key":"e_1_2_7_83_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-08-2804"},{"key":"e_1_2_7_84_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-fluid-010814-014513"},{"key":"e_1_2_7_85_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406682102"},{"key":"e_1_2_7_86_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.8.2442"},{"key":"e_1_2_7_87_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.5.1533.005k01_1533_1540"},{"key":"e_1_2_7_88_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-08-3122"},{"key":"e_1_2_7_89_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-01-407262"},{"key":"e_1_2_7_90_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2000.02034.x"},{"key":"e_1_2_7_91_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.13066"},{"key":"e_1_2_7_92_1","unstructured":"NguyenTH MedvedevN DelceaM GreinacherA. submitted.Anti\u2010platelet factor 4\/polyanion antibodies mediate a new mechanism of autoimmunity."},{"key":"e_1_2_7_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2014.06.028"},{"key":"e_1_2_7_94_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2010.7"}],"container-title":["Journal of Molecular Recognition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmr.2585","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmr.2585","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmr.2585","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T16:43:50Z","timestamp":1696351430000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmr.2585"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,28]]},"references-count":93,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1002\/jmr.2585"],"URL":"https:\/\/doi.org\/10.1002\/jmr.2585","archive":["Portico"],"relation":{},"ISSN":["0952-3499","1099-1352"],"issn-type":[{"value":"0952-3499","type":"print"},{"value":"1099-1352","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,28]]},"article-number":"e2585"}}