{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T02:22:12Z","timestamp":1776824532648,"version":"3.51.2"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T00:00:00Z","timestamp":1479254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Center for Advancing Translational Sciences of the National Institutes of Health","award":["UL1-TR001453"],"award-info":[{"award-number":["UL1-TR001453"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The murine monoclonal antibody LA\u20102 recognizes a clinically protective epitope on outer surface protein (OspA) of <jats:italic>Borrelia burgdorferi<\/jats:italic>, the causative agent of Lyme disease in North America. Human antibody equivalence to LA\u20102 is the best serologic correlate of protective antibody responses following OspA vaccination. Understanding the structural and functional basis of the LA\u20102 protective epitope is important for developing OspA\u2010based vaccines and discovering prophylactic antibodies against Lyme disease. Here, we present a detailed structure\u2010based analysis of the LA\u20102\/OspA interaction interface and identification of residues mediating antibody recognition. Mutations were introduced into both OspA and LA\u20102 on the basis of computational predictions on the crystal structure of the complex and experimentally tested for in vitro binding and borreliacidal activity. We find that Y32 and H49 on the LA\u20102 light chain, N52 on the LA\u20102 heavy chain and residues A208, N228 and N251 on OspA were the key constituents of OspA\/LA\u20102 interface. These results reveal specific residues that may be exploited to modulate recognition of the protective epitope of OspA and have implications for developing prophylactic passive antibodies.<\/jats:p>","DOI":"10.1002\/jmr.2595","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T19:56:06Z","timestamp":1479326166000},"source":"Crossref","is-referenced-by-count":12,"title":["Structural and molecular analysis of a protective epitope of <scp>Lyme<\/scp> disease antigen <scp>OspA<\/scp> and antibody interactions"],"prefix":"10.1002","volume":"30","author":[{"given":"Shivender","family":"Shandilya","sequence":"first","affiliation":[{"name":"Biochemistry and Molecular Pharmacology University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nese","family":"Kurt Yilmaz","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Pharmacology University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Sadowski","sequence":"additional","affiliation":[{"name":"MassBiologics University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ejemel","family":"Monir","sequence":"additional","affiliation":[{"name":"MassBiologics University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary A.","family":"Schiller","sequence":"additional","affiliation":[{"name":"MassBiologics University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"William D.","family":"Thomas","sequence":"additional","affiliation":[{"name":"MassBiologics University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark S.","family":"Klempner","sequence":"additional","affiliation":[{"name":"MassBiologics University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celia A.","family":"Schiffer","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Pharmacology University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"MassBiologics University of Massachusetts Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,16]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"684","article-title":"Lyme disease","volume":"371","author":"Shapiro ED","year":"2014","journal-title":"N Engl J 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