{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:44:53Z","timestamp":1791485093031,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,12,6]],"date-time":"2016-12-06T00:00:00Z","timestamp":1480982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Medical University of Silesia, Katowice, Poland","award":["KNW-1-112\/N\/6\/K"],"award-info":[{"award-number":["KNW-1-112\/N\/6\/K"]}]},{"name":"University of Gdansk, Poland","award":["DS 530-8717-D492-16"],"award-info":[{"award-number":["DS 530-8717-D492-16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The HERV\u2010W family of human endogenous retroviruses represents a group of numerous sequences that show close similarity in genetic composition. It has been documented that some members of HERV\u2010W\u2013derived expression products are supposed to play significant role in humans' pathology, such as multiple sclerosis or schizophrenia. Other members of the family are necessary to orchestrate physiological processes (eg, ERVWE1 coding syncytin\u20101 that is engaged in syncytiotrophoblast formation). Therefore, an assay that would allow the recognition of particular form of HERV\u2010W members is highly desirable. A peptide nucleic acid (PNA)\u2013mediated technique for the discrimination between multiple sclerosis\u2010associated retrovirus and ERVWE1 sequence has been developed. The assay uses a PNA probe that, being fully complementary to the ERVWE1 but not to multiple sclerosis\u2010associated retrovirus (MSRV) template, shows high selective potential. Single\u2010stranded DNA binding protein facilitates the PNA\u2010mediated, sequence\u2010specific formation of strand invasion complex and, consequently, local DNA unwinding. The target DNA may be then excluded from further analysis in any downstream process such as single\u2010stranded DNA\u2010specific exonuclease action. Finally, the reaction conditions have been optimized, and several PNA probes that are targeted toward distinct loci along whole HERV\u2010W <jats:italic>env<\/jats:italic> sequences have been evaluated. We believe that PNA\/single\u2010stranded DNA binding protein\u2013based application has the potential to selectively discriminate particular HERV\u2010W molecules as they are at least suspected to play pathogenic role in a broad range of medical conditions, from psycho\u2010neurologic disorders (multiple sclerosis and schizophrenia) and cancers (breast cancer) to that of an auto\u2010immunologic background (psoriasis and lupus erythematosus).<\/jats:p>","DOI":"10.1002\/jmr.2600","type":"journal-article","created":{"date-parts":[[2016,12,6]],"date-time":"2016-12-06T08:53:27Z","timestamp":1481014407000},"source":"Crossref","is-referenced-by-count":6,"title":["The application of strand invasion phenomenon, directed by peptide nucleic acid (<scp>PNA<\/scp>) and single\u2010stranded <scp>DNA<\/scp> binding protein (<scp>SSB<\/scp>) for the recognition of specific sequences of human endogenous retroviral <scp>HERV\u2010W<\/scp> family"],"prefix":"10.1002","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9081-0984","authenticated-orcid":false,"given":"Grzegorz","family":"Machnik","sequence":"first","affiliation":[{"name":"Department of Internal Medicine and Clinical Pharmacology, School of Medicine in Katowice Medical University of Silesia  Medyk\u00f3w 18 40\u2010760 Katowice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0141ukasz","family":"Bu\u0142dak","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Clinical Pharmacology, School of Medicine in Katowice Medical University of Silesia  Medyk\u00f3w 18 40\u2010760 Katowice Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaros\u0142aw","family":"Ruczy\u0144ski","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry University of Gda\u0144sk  Wita Stwosza 63 80\u2010308 Gda\u0144sk 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