{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T05:01:30Z","timestamp":1781240490233,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,30]],"date-time":"2017-08-30T00:00:00Z","timestamp":1504051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Formation of the deposits of protein aggregates\u2014amyloid fibrils in an intracellular and intercellular space\u2014is common to a large group of amyloid\u2010associated disorders. Among the approaches to develop of therapy of such disorders is the use of agents preventing protein fibrillization. Polyaromatic complexes\u2014porphyrins and phthalocyanines\u2014are known as compounds possessing anti\u2010fibrillogenic activity.<\/jats:p><jats:p>Here, we explore the impact of related macrocyclic complexes\u2014phthalocyanines (Pc) and octaphenyl porphyrazines (Pz) of Mg and Zn\u2014on aggregation of amyloidogenic protein insulin. Pz complexes are firstly reported as compounds able to affect protein fibrillization.<\/jats:p><jats:p>The effect of Pc and Pz complexes on the kinetics and intensity of insulin aggregation was studied by the fluorescent assay using amyloid sensitive cyanine dye. This has shown the impact of metal ion on the anti\u2010fibrillogenic properties of macrocyclic complexes\u2014the effect on the fibrillization kinetics of Mg\u2010containing compounds is much more pronounced comparing to that of Zn analogues.<\/jats:p><jats:p>Scanning electron microscopy experiments have demonstrated that filamentous fibrils are the main product of aggregation both for free insulin and in the presence of macrocyclic complexes. However, those fibrils are distinct by their length and proneness to lateral aggregation. The Pc complexes cause the increase in variation of fibrils length 0.9 to 2.7\u00a0nm in opposite to 1.4 to 2.0\u00a0nm for free insulin, whereas Pz complexes cause certain shortening of the fibrils to 0.8 to 1.6\u00a0nm.<\/jats:p><jats:p>The averaged size of the fibrils population was estimated by dynamic light scattering; it correlates with the size of single fibrils detected by scanning electron microscopy.<\/jats:p>","DOI":"10.1002\/jmr.2660","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T08:22:55Z","timestamp":1504167775000},"source":"Crossref","is-referenced-by-count":6,"title":["Activity of Zn and Mg phthalocyanines and porphyrazines in amyloid aggregation of insulin"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8305-9398","authenticated-orcid":false,"given":"V.","family":"Kovalska","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology and Genetics NASU  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Chernii","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Genetics NASU  Kyiv Ukraine"},{"name":"V.I. Vernadskii Institute of General and Inorganic Chemistry NASU  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Losytskyy","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Genetics NASU  Kyiv Ukraine"},{"name":"V.I. Vernadskii Institute of General and Inorganic Chemistry NASU  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ostapko","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry PAS  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Tretyakova","sequence":"additional","affiliation":[{"name":"V.I. Vernadskii Institute of General and Inorganic Chemistry NASU  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Gorski","sequence":"additional","affiliation":[{"name":"Institute of Physical Chemistry PAS  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Chernii","sequence":"additional","affiliation":[{"name":"V.I. Vernadskii Institute of General and Inorganic Chemistry NASU  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Yarmoluk","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Genetics NASU  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.101304.123901"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005566"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(98)80016-X"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.5b00507"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0902603106"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01290-8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2014.10.024"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2011.10.083"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.7124\/bc.00083C"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.6060\/mhc131160c"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/jr9370000929"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520291003648466"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(93)81312-2"},{"key":"e_1_2_6_15_1","first-page":"131","article-title":"Conformational constraints for amyloid fibrillation: the importance of being unfolded","volume":"2004","author":"Uversky V","year":"1698","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2006.11.008"},{"key":"e_1_2_6_17_1","article-title":"The impact of binding of macrocyclic metal complexes on amyloid fibrillization of insulin and lysozyme","author":"Kovalska V","journal-title":"J Mol Recognit"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6118"},{"key":"e_1_2_6_19_1","volume-title":"The Theory of Polymer Dynamics","author":"Doi M","year":"1995"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054541"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2013.06.009"},{"key":"e_1_2_6_22_1","unstructured":"WeissM SteinerD PhilipsonL.Insulin biosynthesis secretion structure and structure\u2010activity relationships."},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0910080106"}],"container-title":["Journal of Molecular Recognition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmr.2660","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmr.2660","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T08:46:58Z","timestamp":1695026818000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmr.2660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,30]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1002\/jmr.2660"],"URL":"https:\/\/doi.org\/10.1002\/jmr.2660","archive":["Portico"],"relation":{},"ISSN":["0952-3499","1099-1352"],"issn-type":[{"value":"0952-3499","type":"print"},{"value":"1099-1352","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,30]]},"article-number":"e2660"}}