{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:56Z","timestamp":1753869416316,"version":"3.41.2"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,6]],"date-time":"2017-10-06T00:00:00Z","timestamp":1507248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Social Development Project (Health) Guidance Program","award":["KSZ1301"],"award-info":[{"award-number":["KSZ1301"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Stat3 signaling has been recognized as a potential therapeutic target of human ovarian cancer. The signaling is transducted through the peptide\u2010medicated interaction of Stat3 with BET family members Brd2 and Brd4 \u2013\u2013 2 highly homologous proteins involved in differential downstream pathways. Here, we reported a successful design of peptide selectivity between the Brd2 and Brd4. The design resulted in 3 linear peptides SMSLQC<jats:styled-content>X<\/jats:styled-content>YLGVA, QSKVLT<jats:styled-content>X<\/jats:styled-content>SYWGA, and RQCNLG<jats:styled-content>X<\/jats:styled-content>LYMNY with high or moderate selectivity for Brd2 over Brd4 (<jats:italic>S<\/jats:italic>\u00a0=\u00a03.3\u2010fold, 6.8\u2010fold, and 4.2\u2010fold, respectively) as compared with the native Stat3 peptide <jats:sup>281<\/jats:sup>HNLLRI<jats:styled-content>X<\/jats:styled-content>QFLQS<jats:sup>292<\/jats:sup> (<jats:italic>S<\/jats:italic>\u00a0=\u00a02.5\u2010fold). Structural analysis revealed that peptide N\u2010terminus and hydrogen bonds play important roles in the peptide interaction stability and specificity with Brd2 and Brd4. This study would help to establish an integrated in silico\u2010in vitro method for rational molecular design of peptide ligand selectivity between homologous protein receptors.<\/jats:p>","DOI":"10.1002\/jmr.2679","type":"journal-article","created":{"date-parts":[[2017,10,6]],"date-time":"2017-10-06T07:06:52Z","timestamp":1507273612000},"source":"Crossref","is-referenced-by-count":5,"title":["Molecular design of Stat3\u2010derived peptide selectivity between BET proteins Brd2 and Brd4 in ovarian cancer"],"prefix":"10.1002","volume":"31","author":[{"given":"Lixia","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics the First People's Hospital of Kunshan  Kunshan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3779-2382","authenticated-orcid":false,"given":"Xi","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pharmacy the Affiliated Hospital of Nantong University  Dongtai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3818"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.197"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204990"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncoscience.26"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2013.01.023"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1158\/1535-7163.MCT-16-0606","article-title":"Suppression of the growth and invasion of human head and neck squamous cell carcinomas via regulating STAT3 signaling and the miR\u201021\/\u03b2\u2010catenin axis with HJC0152","volume":"16","author":"Wang Y","year":"2017","journal-title":"Mol Cancer Ther"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2734"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.12.022"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130376"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.6b01761"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/minf.201600088"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiolchem.2016.05.009"},{"key":"e_1_2_7_14_1","article-title":"Staurosporine scaffold\u2010based rational discovery of the wild\u2010type sparing reversible inhibitors of EGFR T790M gatekeeper mutant in lung cancer with analog\u2010sensitive kinase technology","author":"Song X","year":"2017","journal-title":"J Mol Recognit"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10989-015-9501-9"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.22488"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.10349"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.445869"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6MB00297H"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/minf.201300064"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci500522v"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2017.1360327"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20290"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2012.03.030"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.464397"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9991(77)90098-5"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2015.1032936"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/ci3001919"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05998"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21109"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5MB00800J"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.6b00673"}],"container-title":["Journal of Molecular Recognition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmr.2679","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmr.2679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T06:13:01Z","timestamp":1695967981000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmr.2679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,6]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1002\/jmr.2679"],"URL":"https:\/\/doi.org\/10.1002\/jmr.2679","archive":["Portico"],"relation":{},"ISSN":["0952-3499","1099-1352"],"issn-type":[{"type":"print","value":"0952-3499"},{"type":"electronic","value":"1099-1352"}],"subject":[],"published":{"date-parts":[[2017,10,6]]},"article-number":"e2679"}}