{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:26:39Z","timestamp":1761395199928,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T00:00:00Z","timestamp":1520380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2017CFB149"],"award-info":[{"award-number":["2017CFB149"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this work, the interaction of an anti\u2010HIV drug lamivudine and human serum albumin (HSA) was studied by multispectroscopic and molecular modeling methods. The fluorescence emission spectra showed that the fluorescence of HSA was quenched by lamivudine through static mechanism with HSA\u2010lamivudine complex produced at ground state. According to the binding equilibriums observed at 4 different temperatures, the number of binding site, binding constant, enthalpy change, entropy change, and Gibbs free energy change of the interaction were calculated. The results indicated that there was only 1 main binding site under present concentration condition, and then, the location of this binding site was ascertained by molecular probe experiments using warfarin and ibuprofen as site markers. The interaction was a spontaneous and exothermic process. Hydrogen bonds and van der Waals force were the major power that fixed lamivudine on Sudlow's site I in subdomain IIA of HSA molecule. The distance between donor and acceptor was determined by F\u00f6rster's nonradiative fluorescence resonance energy transfer theory. Circular dichroism spectra exhibited the alteration of HSA's secondary structures. Molecular modeling investigation revealed the structure of HSA\u2010lamivudine complex, including the conformation of lamivudine in binding site, the amino residues close to lamivudine, and the interaction forces between receptor and ligand. The study may be beneficial to therapists in understanding the distribution of lamivudine in vivo and explaining its drug\u2010resistant mechanism in clinical diagnosis.<\/jats:p>","DOI":"10.1002\/jmr.2705","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T08:44:41Z","timestamp":1520412281000},"source":"Crossref","is-referenced-by-count":10,"title":["Study on the molecular recognition action of lamivudine by human serum albumin"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1739-0124","authenticated-orcid":false,"given":"Hua\u2010xin","family":"Zhang","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Drug Synthesis and Optimization Jingchu University of Technology  Jingmen Hubei People's Republic of China"},{"name":"Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Hubei University  Wuhan Hubei People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Hubei University  Wuhan Hubei People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing\u2010hua","family":"Xia","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Drug Synthesis and Optimization Jingchu University of Technology  Jingmen Hubei People's Republic of China"},{"name":"Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials Hubei University  Wuhan Hubei People's Republic of 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