{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:28:07Z","timestamp":1789147687012,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,4,6]],"date-time":"2018-04-06T00:00:00Z","timestamp":1522972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","award":["01(2769)\/13\/EMR\u2010II"],"award-info":[{"award-number":["01(2769)\/13\/EMR\u2010II"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Epitope imprinting is a promising technique for fabrication of novel diagnostic tools. In this study, an epitope imprinted methodology for recognition of target epitope sequence as well as targeted protein infused by bacterial infection in blood samples of patients suffering from brain fever is developed. Template sequence chosen is a ferric iron binding fbp A protein present in <jats:italic>Neisseria meningitidis<\/jats:italic> bacteria. To orient the imprinting template peptide sequence on gold surface of electrochemical quartz crystal microbalance (EQCM), thiol chemistry was utilized to form the self\u2010assembled monolayer on EQCM electrode. Here, synergistic effects induced by various noncovalent interactions extended by multiple monomers (3\u2010sulfopropyl methacrylate potassium\u2010salt and benzyl methacrylate) were used in fabricating the imprinting polymeric matrix with additional firmness provided by <jats:italic>N<\/jats:italic>,<jats:italic>N<\/jats:italic>\u2010methylene\u2010<jats:italic>bis<\/jats:italic>\u2010acrylamide as cross\u2010linker and azo\u2010isobutyronitrile as initiator. Extraction of template molecule was carried out with phosphate buffer solution. After extraction of epitope molecules from the polymeric film, epitope molecularly imprinted polymeric films were fabricated on EQCM electrode surface. Nonimprinted polymers were also synthesized in the similar manner without epitope molecule. Detection limit of epitope molecularly imprinted polymers and imprinting factor (epitope molecularly imprinted polymers\/nonimprinted polymers) was calculated 1.39\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup> and 12.27 respectively showing high binding capacity and specific recognition behavior toward template molecule. Simplicity of present method would put forward a fast, facile, cost\u2010effective diagnostic tool for mass health care.<\/jats:p>","DOI":"10.1002\/jmr.2709","type":"journal-article","created":{"date-parts":[[2018,4,9]],"date-time":"2018-04-09T20:20:13Z","timestamp":1523305213000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Epitope imprinting of iron binding protein of <i>Neisseria meningitidis<\/i> bacteria through multiple monomers imprinting approach"],"prefix":"10.1002","volume":"31","author":[{"given":"Neha","family":"Gupta","sequence":"first","affiliation":[{"name":"Department of Chemistry, MMV Banaras Hindu University  Varanasi 221005 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roop Shikha","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Institute of Science Banaras Hindu University  Varanasi 221005 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