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However, the toxicity of QDs to organisms has attracted wide attention. In our research, we introduced an in vitro method to study the molecular mechanisms for the structure and activity alterations of <jats:styled-content style=\"fixed-case\"><jats:italic>Candida rugosa<\/jats:italic><\/jats:styled-content> lipase (CRL) with the binding of 3\u2010mercaptopropionic acid\u2010capped CdTe QDs. Multiple spectroscopic methods, isothermal titration calorimetry, and enzyme activity measurements were used in this paper. QDs statically quenched the intrinsic fluorescence of CRL with the quenching constant decreases from 2.46\u00a0\u00d7\u00a010<jats:sup>13<\/jats:sup> to 1.64\u00a0\u00d7\u00a010<jats:sup>13<\/jats:sup>\u00a0L\u00a0mol<jats:sup>\u22121<\/jats:sup>\u00a0second<jats:sup>\u22121<\/jats:sup> (298 to 310\u00a0K). It binds to CRL through hydrophobic force with 1 binding site, unfolding and loosening the skeleton and changed its secondary structure. Rather than aggregating on the surface, it enters the pocket of the CRL to interact with Ser\u2010209 (2.43\u00a0\u00c5) and the residues surrounding Ser\u2010209, making the catalytic triad more exposed. Furthermore, the activity of CRL was inhibited by approximately 15%. This work demonstrates that 3\u2010mercaptopropionic acid\u2010capped CdTe QDs may cause negative effects to CRL and obtains a molecular mechanism on QD\u2010induced toxicity to proteins in vitro.<\/jats:p>","DOI":"10.1002\/jmr.2712","type":"journal-article","created":{"date-parts":[[2018,4,14]],"date-time":"2018-04-14T22:32:05Z","timestamp":1523745125000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The binding interaction between cadmium\u2010based, aqueous\u2010phase quantum dots with <scp><i>Candida rugosa<\/i><\/scp> lipase"],"prefix":"10.1002","volume":"31","author":[{"given":"Lining","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Environmental Science and Engineering Shandong University, China\u2010America CRC for Environment &amp; Health, Shandong Province  Jinan P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology for Environment and Health, Shandong Analysis and Test Center Qilu University of Technology (Shandong Academy of Sciences)  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7264-0531","authenticated-orcid":false,"given":"Rutao","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering Shandong University, China\u2010America CRC for Environment &amp; Health, Shandong Province  Jinan P. R. 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