{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T20:41:37Z","timestamp":1783024897581,"version":"3.54.6"},"reference-count":56,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T00:00:00Z","timestamp":1524787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007837","name":"Universit\u00e4t Bremen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mechanical properties of myofibroblasts play a key role in Dupuytren's disease. Here, we used atomic force microscopy to measure the viscoelastic properties of 3 different types of human primary fibroblasts derived from a same patient: normal and scar dermal fibroblasts and palmar fascial fibroblasts from Dupuytren's nodules. Different stiffness hydrogels (soft ~1\u00a0kPa and stiff ~ 50\u00a0kPa) were used as cell culture matrix to mimic the mechanical properties of the natural tissues, and atomic force microscopy step response force curves were used to discriminate between elastic and viscous properties of cells. Since transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) is known to induce expression of \u03b1\u2013smooth muscle actin positive stress fibers in myofibroblasts, we investigated the behavior of these fibroblasts before and after applying TGF\u2010\u03b21. Finally, we performed an <jats:italic>in vitro<\/jats:italic> cell motility test, the wound healing or scratch assay, to evaluate the migratory properties of these fibroblasts. We found that (1) Dupuytren's fibroblasts are stiffer than normal and scar fibroblasts, the elastic modulus E ranging from 4.4, 2.1, to 1.8\u00a0kPa, for Dupuytren's, normal and scar fibroblasts, respectively; (2) TGF\u2010\u03b21 enhances the level of \u03b1\u2013smooth muscle actin expression and thus cell stiffness in Dupuytren's fibroblasts (E, ~6.2\u00a0kPa); (3) matrix stiffness influences cell mechanical properties most prominently in Dupuytren's fibroblasts; and (4) Dupuytren's fibroblasts migrate slower than the other fibroblasts by a factor of 3. Taking together, our results showed that mechanical and migratory properties of fibroblasts might help to discriminate between different pathological conditions, helping to identify and recognize specific cell phenotypes.<\/jats:p>","DOI":"10.1002\/jmr.2719","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T10:49:49Z","timestamp":1524826189000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Mechanical and migratory properties of normal, scar, and Dupuytren's fibroblasts"],"prefix":"10.1002","volume":"31","author":[{"given":"Prem Kumar","family":"Viji Babu","sequence":"first","affiliation":[{"name":"Institute of Biophysics University of Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"Rianna","sequence":"additional","affiliation":[{"name":"Institute of Biophysics University of Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gazanfer","family":"Belge","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Chemistry University of Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula","family":"Mirastschijski","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive, and Aesthetic Surgery, Klinikum Bremen\u2010Mitte, and Wound Repair Unit, CBIB University of Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8744-4541","authenticated-orcid":false,"given":"Manfred","family":"Radmacher","sequence":"additional","affiliation":[{"name":"Institute of Biophysics University of Bremen  Bremen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13069-016-0046-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9290(06)84993-6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198163050-00014"},{"issue":"4","key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S0749-0712(21)00907-0","article-title":"The myofibroblast in Dupuytren's contracture","volume":"7","author":"Rudolph R","year":"1991","journal-title":"Hand Clin"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1297-3203(00)73455-X"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0363-5023(05)80067-X"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200704042"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.122.1.103"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.142.3.873"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm809"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1994.1290"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1109\/51.582176"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(96)79602-9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M701867200"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0827"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl4013248"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2013.0125"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-538-5_2"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E04-06-0485"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76614-8"},{"key":"e_1_2_9_22_1","doi-asserted-by":"crossref","unstructured":"RiannaC RadmacherM.Cell mechanics as a marker for diseases: biomedical applications of AFM. 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