{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T06:05:58Z","timestamp":1783058758597,"version":"3.54.6"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T00:00:00Z","timestamp":1537747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004083","name":"Ministry of Science ICT and Future Planning","doi-asserted-by":"publisher","award":["2015M3C8A6A06012737"],"award-info":[{"award-number":["2015M3C8A6A06012737"]}],"id":[{"id":"10.13039\/501100004083","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endocrine\u2010disrupting chemicals are a major public health problem throughout the world. In the human body, these compounds functionalize the same as sexual hormones, inducing precocious puberty, gynecomastia, etc. To help prevent this occurrence, a simple detection system is needed. In this study, a nonylphenol ethoxylate (NPE)\u2010specific aptamer was selected by reduced graphene oxide\u2010systematic evolution of ligands by exponential enrichment. A random ssDNA library was incubated with rGO for adsorption, followed by elution with the target molecule. As a result of screening, a DNA aptamer was found that specifically bounds to the target with high binding affinity (<jats:italic>K<\/jats:italic><jats:sub><jats:italic>d<\/jats:italic><\/jats:sub>\u00a0=\u00a0100.9\u00a0\u00b1\u00a013.2\u00a0nM) and had a low limit of detection (LOD\u00a0=\u00a0696\u00a0pM). Furthermore, this NPE\u2010binding aptamer bounds selectively to the target. Characterization of the aptamer was confirmed by measuring the fluorescence signal recovery from rGO. In addition, detection of NPE was performed with several water samples, and the detection accuracy was 100\u00a0\u00b1\u00a010%. From these results, we expect that this aptamer could be applied to an on\u2010site detection system for NPE in industrial sites or domestic fields.<\/jats:p>","DOI":"10.1002\/jmr.2764","type":"journal-article","created":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T05:19:05Z","timestamp":1537852745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Development of a ssDNA aptamer system with reduced graphene oxide (rGO) to detect nonylphenol ethoxylate in domestic detergent"],"prefix":"10.1002","volume":"32","author":[{"given":"A\u2010Ru","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Chemistry and Research Institute of Natural Sciences Hanyang University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na\u2010Reum","family":"Ha","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Research Institute of Natural Sciences Hanyang University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u2010Pil","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Research Institute of Natural Sciences Hanyang University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang\u2010Heon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Research Institute of Natural Sciences Hanyang University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8548-2915","authenticated-orcid":false,"given":"Moon\u2010Young","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Research Institute of Natural Sciences Hanyang University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,24]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"World Health Organization.Global assessment of the state\u2010of\u2010the\u2010science of EDCs.http:\/\/www.who.int\/ipcs\/publications\/en\/ch2.pdf. 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