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In this report, we have mined and classified the proteins involved in pathways unique to\n                    <jats:italic>Ott<\/jats:italic>\n                    by using high\u2010throughput computational techniques. The 12 metabolic pathways were found to be unique to the pathogen. Forty\u2010six proteins were reported to be essential for the pathogen's survival and non\u2010homologous to the humans. The proteins were categorized into different classes, ie, enzymes, transporters, DNA\u2010binding, secretory, and outer membrane proteins. Further,\n                    <jats:italic>in silico<\/jats:italic>\n                    analysis of 46 proteins showed that 25 proteins were suitable therapeutic targets with known druggable properties. The structural modeling of B3CSG3 (MurA) protein was carried out and catalytic site essential for its functioning was analyzed. Virtual screening of chemical compounds was performed against modeled structure. The docking study by AutodockVina reported compound from PubChem with CID: 16036947 as best and potential inhibitor by means of docking score and binding affinity. The reliability and stability of the MurA\u201016036947 complex were confirmed with molecular dynamics simulation. The report will provide insight to understand the mechanism of pathogenesis of\n                    <jats:italic>Ott<\/jats:italic>\n                    and instigate the development of effective treatment strategies against this disease.\n                  <\/jats:p>","DOI":"10.1002\/jmr.2766","type":"journal-article","created":{"date-parts":[[2018,10,21]],"date-time":"2018-10-21T19:40:47Z","timestamp":1540150847000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Targeting metabolic pathways proteins of Orientia tsutsugamushi using combined hierarchical approach to combat scrub typhus"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9911-9364","authenticated-orcid":false,"given":"Dixit","family":"Sharma","sequence":"first","affiliation":[{"name":"Centre for Computational Biology and Bioinformatics, School of Life Sciences Central University of Himachal Pradesh Shahpur, District\u2010Kangra Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Sharma","sequence":"additional","affiliation":[{"name":"Centre for Computational Biology and Bioinformatics, School of Life Sciences Central University of Himachal Pradesh Shahpur, District\u2010Kangra Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailender Kumar","family":"Verma","sequence":"additional","affiliation":[{"name":"Centre for Computational Biology and Bioinformatics, School of Life Sciences Central University of Himachal Pradesh Shahpur, District\u2010Kangra Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birbal","family":"Singh","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Veterinary Research Institute, Regional Station Palampur Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.13-0064"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1603657"},{"key":"e_1_2_8_4_1","first-page":"170","article-title":"Approaches to vaccines against Orientia tsutsugamushi","volume":"2","author":"Valbuena G","year":"2012","journal-title":"Front Cell Infect Microbiol"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003971"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/516990"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1709.100960"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01526-10"},{"key":"e_1_2_8_9_1","first-page":"954","article-title":"Scrub typhus","volume":"53","author":"Mahajan SK","year":"2005","journal-title":"J Assoc Physicians India"},{"key":"e_1_2_8_10_1","article-title":"Scrub typhus: an emerging threat","author":"Chakraborty S","year":"2017","journal-title":"Indian J Dermatol"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1610.100456"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-016-0186-x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2301.161038"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-015-1428-x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4103\/0028-3886.107919"},{"key":"e_1_2_8_16_1","unstructured":"WHORecommended Surveillance Standards. 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