{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:11:24Z","timestamp":1790759484373,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T00:00:00Z","timestamp":1550016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["16\u201004\u201000609"],"award-info":[{"award-number":["16\u201004\u201000609"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lactoferrin (LF) is an Fe<jats:sup>3+<\/jats:sup>\u2010binding glycoprotein first recognized in milk and then in other epithelial secretions and barrier body fluids to which many different functions have been attributed to LF, including protection from iron\u2010induced lipid peroxidation, immunomodulation, cell growth regulation, DNA and RNA binding, as well as transcriptional activation, \u0435t\u0441. The polyfunctional physiological role of LF is still unclear, but it has been suggested to be responsible for primary defense against microbial and viral infections. Here, we present the first evidence that LF preparations isolated from milk of 18 cows of different breeds possess various levels of metal\u2010dependent DNase and metal\u2010independent RNase activities. For univocal assignment of DNase and RNase activities to cow LF, it was subjected to SDS\u2010PAGE using gels with copolymerized calf thymus DNA or polymeric yeast RNA. In situ analysis was revealed DNase and RNase activities only in the gel zones corresponding to homogeneous LF. In contrast to human LF, cow LF possesses a relatively low cytotoxicity towards human tumor cells. The discovery that cow LF has these activities may contribute to understanding the multiple physiological functions of this extremely polyfunctional protein, including its protective role against microbial and viral infections. The computational spatial model of cow LF complex with DNA was obtained: according to the model positively charged residues of LF contact with DNA.<\/jats:p>","DOI":"10.1002\/jmr.2777","type":"journal-article","created":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T06:30:34Z","timestamp":1550125834000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["DNase and RNase activities of fresh cow milk lactoferrin"],"prefix":"10.1002","volume":"32","author":[{"given":"Svetlana E.","family":"Soboleva","sequence":"first","affiliation":[{"name":"Siberian Division of Russian Academy of Sciences Institute of Chemical Biology and Fundamental Medicine  Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ol'ga D.","family":"Zakharova","sequence":"additional","affiliation":[{"name":"Siberian Division of Russian Academy of Sciences Institute of Chemical Biology and Fundamental Medicine  Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey E.","family":"Sedykh","sequence":"additional","affiliation":[{"name":"Siberian Division of Russian Academy of Sciences Institute of Chemical Biology and Fundamental Medicine  Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikita V.","family":"Ivanisenko","sequence":"additional","affiliation":[{"name":"Siberian Division of Russian Academy of Sciences Institute of Cytology and Genetics  Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina N.","family":"Buneva","sequence":"additional","affiliation":[{"name":"Siberian Division of Russian Academy of Sciences Institute of Chemical Biology and Fundamental Medicine  Novosibirsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4988-8923","authenticated-orcid":false,"given":"Georgy 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