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An ideal detection system for pathogen control with excellent sensitivity, high specificity, and time\u2010saving is needed. Here, we reported a method for bacterial detection using gold nanoparticles\u2010mediated fluorescent \u201cchemical nose\u201d sensors (GFCEs). The technique consists of gold nanoparticles\u2010coated magnetic particle using benzaldehyde, octyl aldehyde, and pyrimidine\u20104\u2010formaldehyde modified, respectively. And these positively charged nanocompound interacting with three different fluorescent proteins (FPs) to form three kinds of GFCEs, respectively, named GFCE1, GFCE2, and GFCE3. Upon binding with pathogenic cells, functionalized gold nanoparticles could identify patches on hydrophobic\/functional surfaces of microorganisms, and self\u2010assemble with living bacteria by complementary electrostatic interactions. The binding ability between GFCEs and bacteria determines the change of fluorescence response of three FPs from GFCEs. These feature fluorescent level are pathogen\u2010specific, highly repeatable, and can be analyzed by Linear Discriminant Analysis (LDA). The combination of GFCE1 and GFCE2 has the best performance when detecting pathogens with concentrations of 10<jats:sup>6<\/jats:sup>\u2009cfu\u2009mL<jats:sup>\u22121<\/jats:sup>. The first discriminant within 15\u2009minutes is 93.8%, which could be used for subsequent identification of unknown samples. The commonly applicable system provides a simple way for the rapid bacterial detection without preprocessing procedures.<\/jats:p>","DOI":"10.1002\/jmr.2919","type":"journal-article","created":{"date-parts":[[2021,6,17]],"date-time":"2021-06-17T08:17:38Z","timestamp":1623917858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Gold nanoparticles\u2010mediated fluorescent chemical nose sensor for pathogenic diagnosis and phenotype"],"prefix":"10.1002","volume":"34","author":[{"given":"Yuanyuan","family":"Shen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University  Haikou PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feifei","family":"Lei","sequence":"additional","affiliation":[{"name":"School of Computer Science and Cyberspace Security Hainan University  Haikou PR 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