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Peritoneal macrophages were stimulated with VGL for dose\u2010dependent gene expression and release of TNF\u2010\u03b1. In vivo results showed that VGL (1\u00a0mg\/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6\u00a0hours after induction. In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF\u2010\u03b1 dosage (mean\u2009\u00b1\u2009SEM (<jats:italic>n<\/jats:italic>\u00a0=\u00a06), analysis of variance, and Bonferroni's test (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin\u2010induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF\u2010\u03b1 gene expression (RT\u2010PCR) and release from peritoneal macrophages. Thus, upon lectin\u2010glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF\u2010\u03b1 and TLR and\/or TNFR receptor pathways.<\/jats:p>","DOI":"10.1002\/jmr.2922","type":"journal-article","created":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T10:37:17Z","timestamp":1623839837000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Vatairea guianensis<\/i> lectin stimulates changes in gene expression and release of <scp>TNF<\/scp>\u2010\u03b1 from rat peritoneal macrophages via glycoconjugate binding"],"prefix":"10.1002","volume":"34","author":[{"given":"Gabriela Fernandes Oliveira","family":"Marques","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Universidade Estadual do Cear\u00e1  Fortaleza 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