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An innovative protocol was proposed for the green synthesis of gold nanoparticle\u2010supported dimethylglyoxime (AuNPs@DMGO) using one\u2010step electrogeneration method. Also, the application of AuNPs@DMGO for the sensitive quantification of<jats:italic>\u03b1<\/jats:italic>\u2010Synuclein (SNCA) protein and its biomedical analysis. So, an innovative sandwich immunosensor was designed for the sensitive identification of SNCA antigen in an aqueous solution. The gold nanoparticles (AuNPs) were decorated on the surface of the glassy carbon electrode by chronoamperometry technique to provide appropriate immobilization surface with a large number of active sites for immobilization of specific biotinylated antibody (Ab1) and against SNCA protein. Then, the sandwich\u2010type immuno\u2010platform was completed by the attachment of secondary antibody (HRP conjugated Ab [Ab2]) to the primary complexes on the surface of the electrode. For the first time, \u03b1\u2010Synuclein protein was measured with an acceptable linear range of 4\u201364\u2009ng\/mL and a lower limit of quantification of 4\u2009ng\/mL. Benefiting from the simplicity and high sensitivity, the proposed method shows a potential of employment in clinical applications and high\u2010throughput screening of Parkinson's disease using POC.<\/jats:p>","DOI":"10.1002\/jmr.2952","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T11:21:15Z","timestamp":1641381675000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Sensitive electrochemical recognition of<scp><i>\u03b1<\/i>\u2010synuclein<\/scp>protein in human plasma samples using bioconjugated gold nanoparticles: An innovative immuno\u2010platform to assist in the early stage identification of Parkinson's disease by biosensor technology"],"prefix":"10.1002","volume":"36","author":[{"given":"Esmaeil Darvish","family":"Aminabad","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Natural Sciences University of Tabriz Tabriz 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