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In this study, latex obtained from this plant was fractioned using reversed\u2010phase chromatography, and the fractions were then screened for peptides. A 755\u2009g\/mol peptide was obtained, and MS\/MS analyses indicated it had a cyclic sequence (Pro\u2010Leu\u2010Gly\u2010Val\u2010Leu\u2010Leu\u2010Tyr). This peptide sequence was present in the<jats:italic>Jatropha<\/jats:italic>genome database, and an identity value of 90.71%, an E\u2010value of 0.0, and a score of 883 with NO\u2010associated protein 1\/chloroplastic\/mitochondria of<jats:italic>Jatropha curcas<\/jats:italic>were obtained from the NCBI nonredundant protein sequence (nr) database. Molecular docking analyses performed with the peptide against a metalloendopeptidase belonging to<jats:italic>Crotalus adamanteus<\/jats:italic>snake venom suggested the cyclic peptide establishes favorable interactions with the catalytic site of the enzyme. Therefore, it could inhibit enzyme catalysis. This belief was corroborated by the formation of 6 hydrogen bonds with the linear form of the peptide. Tighter complexation of the cyclic form (41\u2009kcal\/mol more energetic) revealed better spatial blocking. The linear form outperformed the cyclic form in complexing the required energy, recruiting more catalytic residues (6\/2), and in establishing more hydrogen bonds (6\/3). However, cyclic folding provided a more significant spatial block within the catalytic site. The set of results suggests that the cycle peptide, here called Jatromollistatin, which was previously described as jatrophidin and pohlianin A in two other species of Jatropha, is a promising candidate to inhibit venom proteases. This belief is corroborated by the topical use of the latex for initial treatment of snakebites.<\/jats:p>","DOI":"10.1002\/jmr.2957","type":"journal-article","created":{"date-parts":[[2022,2,26]],"date-time":"2022-02-26T12:58:55Z","timestamp":1645880335000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insights on the inhibition properties of<i>Jatromollistatin<\/i>(a cyclic heptapeptide) against<i>Crotalus adamanteus<\/i>metalloendopeptidase using molecular docking analysis"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6895-6365","authenticated-orcid":false,"given":"Thiago Lustosa","family":"Juc\u00e1","sequence":"first","affiliation":[{"name":"Experimental Biology Centre (NUBEX) University of Fortaleza (UNIFOR) Fortaleza Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcio 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