{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:30:31Z","timestamp":1778668231200,"version":"3.51.4"},"reference-count":21,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2004,2,3]],"date-time":"2004-02-03T00:00:00Z","timestamp":1075766400000},"content-version":"vor","delay-in-days":3320,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immobilized metal\u2010ion affinity partitioning (IMAP) is shown to be useful as a preliminary screening test and for the separation of different cell populations based upon recognition of the differences in the proteins on cell surfaces. The feasibility of using IMAP to segregate a spectrum of normal human cells (red blood cells, lymphocytes and fibroblasts) from their counterpart pathological cells has been demonstrated.<\/jats:p><jats:p>A clear segregation between normal and sickle\u2010cell anemia red blood cells (RBC), or malaria (<jats:italic>Plasmodium vivax<\/jats:italic>) infected RBCs was obtained. Further, the partition differences were found to depend on the nature and the concentration of metal used.<\/jats:p><jats:p>Cells form breast cancer and those from the lugn adenocarcinoma showed differences in their partition pattern as compared to normal fibroblasts when PEG\u2013IDA\u2013M(II) was added to the phase system. Maximum differences between the three cell populations were observed in the presence of 10% PEG\u2010IDA\u2010Ni(II).<\/jats:p><jats:p>Normal lymphocytes and Burkitt's lymphoma cells (Raji nad Namalwa cell lines) were shown to partition differently in the presence of PEG\u2013IDA\u2013M(II) in the phase system. Normal lymphocytes could be distinguished from the Burkitt's lymphoma cell lines in all three phases (top, interface and bottom), in the presence of 10% PEG\u2013IDA\u2013Ni(II) in the system.<\/jats:p><jats:p>These differences in the partition behavior could mainly be attributed to the density, surface exposure and micro\u2010environment of histidine residues of cell membrane\u2010associated proteins. These data, along with those obtained for normal and pathological human cells show that IMAP could be a simple and versatile tool for the segregation and study of cells.<\/jats:p>","DOI":"10.1002\/jmr.300080114","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T10:35:27Z","timestamp":1117362927000},"page":"77-84","source":"Crossref","is-referenced-by-count":9,"title":["Segregation of normal and pathological human red blood cells, lymphocytes and fibroblasts by immobilized metal\u2010ion affinity partitioning"],"prefix":"10.1002","volume":"8","author":[{"given":"E.","family":"Nanak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Vijayalakshmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. C.","family":"Chadha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/177771a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","unstructured":"Birkenmeier G. Vijayalakshmi M. A. Stigbrand T.andKopperschl\u00e4ger J.(1991). Immobilized metal ion affinity partitioning a method combining metal\u2010protein interaction and partitioning of proteins in aqueous two\u2010phas systems.J. Chromatogr.267\u2013277.","DOI":"10.1016\/S0021-9673(01)83935-2"},{"issue":"97","key":"e_1_2_1_4_1","article-title":"Separation of leucocytes from blood and bone marrow","volume":"21","author":"Boyum A.","year":"1968","journal-title":"Scan. J. Lab. Invest."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1991.390"},{"key":"e_1_2_1_6_1","unstructured":"Fisher D. Baldwin J. Morrison J. O'Reilly R. Ahkong F. Q. Tilcock C.andLucy J. A.(1993). Phase partitioning detects translocation of phosphatidyl serine from the inner to the outer monolayer of erythrocytes. Abstract from the 8th International Conference on Partitioning in Aqueous Two Phase Systems 22\u201329 August Leigpzig (Germany)."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(91)90041-E"},{"key":"e_1_2_1_8_1","first-page":"3","article-title":"Laboratory synthesis of polyethylene glycol derivatives","volume":"25","author":"Harris J. M.","year":"1985","journal-title":"J. Macromol. Sci. Rev. Polym. Chem. 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