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In clinical trial, \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010adrenergic receptor (\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR) has been recognized as a key therapeutic target in cardiovascular disease treatment. So, in this work, we engineered a fusion protein by linking the kinase domain of epidermal growth factor receptor (EGFR) to the C\u2010terminus of \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR, which was subsequently immobilized onto the ibrutinib\u2010modified silica gels. Following column preparation by packing the \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR immobilized silica into a stainless\u2010steel column, the specificity and stability of \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR column were evaluated through the retention behavior analysis of atenolol, esmolol, and metoprolol for characterization. Then, using the \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR column combined with MS\/MS, eight BWCP constituents targeting \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR were screened and identified: 6\u2010hydroxy\u20102\u2010(2\u2010phenylethyl) chromone, taxifolin, myristicin, costunolide, dehydrocostus lactone, gallic acid, 3,4\u2010dihydroxybenzoic acid (DHBA), and catechin. The association constants of above screened compounds with immobilized \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR were (6.75\u2009\u00b1\u20090.05)\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    , (13.2\u2009\u00b1\u20090.60)\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    , (3.82\u2009\u00b1\u20090.18)\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    , (145.13\u2009\u00b1\u20091.50)\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    , (199.77\u2009\u00b1\u20090.30)\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    , (13.8\u2009\u00b1\u20090.04)\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    , (9.30\u2009\u00b1\u20090.14)\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    , (31.4\u2009\u00b1\u20090.76)\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    \u2009M\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively. Molecular docking results indicated that the compound\u2010\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR complexes stabilization primarily involves hydrogen bonding, van der Waals interactions, Pi\u2010alkyl, and Pi\u2010Pi stacking. A survey of DrugBank showed that catechin have well defined effects against cardiovascular diseases. DHBA and taxifolin are potential drug leads under experimental. These confirmed the reliability of the screened compounds by the immobilized \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR. To our knowledge, this work establishes the first systematic identification of BWCP's bioactive components targeting \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010AR, providing a pharmacological foundation for developing novel cardiovascular therapeutics derived from this traditional formulation.\n                  <\/jats:p>","DOI":"10.1002\/jmr.70022","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T05:51:12Z","timestamp":1769147472000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Systematic Identification and Recognition Analysis of Beta1\u2010Adrenergic Receptor Targeting Material Basis in Tibetan Medicine Formula Bawei Chenxiang Powder"],"prefix":"10.1002","volume":"39","author":[{"given":"Lixiang","family":"Chen","sequence":"first","affiliation":[{"name":"Engineering Research Center of Tibetan Medicine Detection Technology, Ministry of 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