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While existing RPA\u2010CRISPR\/Cas12a platforms have demonstrated potential for MRSA detection, most rely on single\u2010gene targets or require multiple Cas enzymes. Here, we have developed a novel dual gene detection strategy that simultaneously detects the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    specific\n                    <jats:italic>femA<\/jats:italic>\n                    gene and the methicillin\u2010resistant\n                    <jats:italic>mecA<\/jats:italic>\n                    gene in a single RPA\u2010CRISPR\/Cas12a reaction. This integrated approach enables clear discrimination between MRSA and methicillin\u2010sensitive\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (MSSA) in just 30\u2009min, with results visualized via both fluorescence and lateral flow strips. The assay exhibited high specificity (no cross\u2010reactivity with common pathogens) and a sensitivity of 10 copies\/\u03bcL, comparable to qPCR. Validation with 39 clinical samples showed 100% concordance with antimicrobial susceptibility testing. Our dual\u2010gene RPA\u2010CRISPR\/Cas12a platform represents a significant advancement in point\u2010of\u2010care MRSA diagnostics, offering enhanced accuracy and operational simplicity.\n                  <\/jats:p>","DOI":"10.1002\/jmr.70035","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T01:04:46Z","timestamp":1776647086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>RPA<\/scp>\n                    Combined With\n                    <scp>CRISPR<\/scp>\n                    \/Cas12a for Rapid and Ultrasensitive Detection Dual\u2010Gene of Methicillin\u2010Resistant\n                    <i>Staphylococcus aureus<\/i>\n                    (\n                    <scp>MRSA<\/scp>\n                    )"],"prefix":"10.1002","volume":"39","author":[{"given":"Lei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Yuebei People's 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