{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T02:59:46Z","timestamp":1785121186215,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201007\u2010PCVI\u20100002\u201001"],"award-info":[{"award-number":["ANR\u201007\u2010PCVI\u20100002\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Immunoglobulin M (IgM) is the first class of antibody produced in response to pathogens. It also plays an important role in regulating the immune response, including in autoimmunity. IgM pentamers consist of an Fc core composed of C\u03bc2, C\u03bc3, and C\u03bc4 domains, as well as 10 Fab domains composed of C\u03bc1 and a variable domain. In this study, we use high\u2010resolution atomic force microscopy (AFM) topography to constrain the reconstruction of pentameric IgM Fc core pseudo\u2010atomic model (without Fabs). Reconstruction is performed in two stages using the AFM\u2010Assembly pipeline. First, we fit the known cryo\u2010EM structure of the inner core of the IgM Fc (C\u03bc3\u2010C\u03bc4) and the known x\u2010ray structure of C\u03bc2 beneath the AFM topographs using the DockAFM tool. Second, the two structural units are assembled to generate a complete pentameric IgM Fc core structure. The experimentally constrained reconstruction of the IgM Fc core reveals a divergence in the assembly of the five C\u03bc2 monomers: the first and last monomers (M1 and M5) are tightly attached to the inner core, while the remaining monomers (M2\u2013M4) are more loosely bound. This reconstruction describes multiple alternative conformations for the attachment of the C\u03bc2 domains, suggesting that the reconstruction protocol integrates molecular flexibility present in molecules individually deposited on the AFM substrate.<\/jats:p>","DOI":"10.1002\/jmr.70041","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T22:08:45Z","timestamp":1784844525000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reconstruction of the Flexible\n                    <scp>IgM<\/scp>\n                    Fc Core Using Atomic Force Microscopy Topography and the\n                    <scp>AFM<\/scp>\n                    \u2010Assembly Pipeline"],"prefix":"10.1002","volume":"39","author":[{"given":"Harinderbir","family":"Kaur","sequence":"first","affiliation":[{"name":"Univ. Grenoble Alpes, CEA, CNRS, IBS  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea J.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, CEA, CNRS, IBS  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Baptiste","family":"Reiser","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, CEA, CNRS, IBS  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai Li","family":"Ling","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, CEA, CNRS, IBS  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8944-2715","authenticated-orcid":false,"given":"Jean\u2010Luc","family":"Pellequer","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, CEA, CNRS, IBS  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"e_1_2_13_2_1","volume-title":"Immunology","author":"Roitt I. 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