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Three of these compounds exhibited stronger binding affinity to FTO, as determined by fluorescence spectroscopy. The results indicate that the binding process is spontaneous and occurs primarily via a static quenching mechanism, driven mainly by hydrogen bonding and van der Waals forces. The interactions of these three lead analogs with FTO were further investigated using UV\u2013visible absorption spectroscopy, circular dichroism spectroscopy, and molecular docking. The docking results are consistent with the thermodynamic data. Among the 28 synthesized derivatives, compounds\n                    <jats:bold>7<\/jats:bold>\n                    ,\n                    <jats:bold>8<\/jats:bold>\n                    , and\n                    <jats:bold>11<\/jats:bold>\n                    exhibited the strongest predicted binding affinities toward FTO and were selected for further spectroscopic and molecular docking studies. In preliminary biological evaluation, compound\n                    <jats:bold>7<\/jats:bold>\n                    showed antiproliferative activity against K562 cells with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 18.11\u2009\u03bcM. This study provides valuable insights into the interaction mechanisms between 2\u2010phenyl\u20101\n                    <jats:italic>H<\/jats:italic>\n                    \u2010benzimidazole analogs and FTO.\n                  <\/jats:p>","DOI":"10.1002\/jmr.70045","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:59:47Z","timestamp":1789430387000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis and Binding Affinity Insights of 2\u2010Phenyl\u20101\n                    <i>H<\/i>\n                    \u2010Benzimidazole Inhibitors Targeting\n                    <scp>FTO<\/scp>\n                    by Spectroscopic Characterization and Molecular Docking"],"prefix":"10.1002","volume":"39","author":[{"given":"Jing","family":"Wang","sequence":"first","affiliation":[{"name":"College of Chemistry Pingyuan Laboratory (Zhengzhou University), Zhengzhou University  Zhengzhou 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