{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T07:53:03Z","timestamp":1761292383358},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,9]],"date-time":"2006-11-09T00:00:00Z","timestamp":1163030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development of high\u2010throughput protein microarrays for rapidly determining antigen\u2010specific T\u2010cell receptor repertoires of diverse T\u2010cell populations can enable comprehensive, broad\u2010based analyses of T\u2010cell responses. Promising applications include medical diagnostics, vaccine development, treatment of autoimmune diseases and detection of potential agents of bioterrorism. In this study, we examined the feasibility of using peptide\/major histocompatibility complex (p\/MHC) microarrays to selectively capture and enumerate antigen\u2010specific T cells. Results are presented for p\/MHC microarrays consisting of a dimeric MHC\u2010immunoglobulin complex, K<jats:sup>b<\/jats:sup>\u2010Ig, loaded with either a cognate or non\u2010cognate peptide for binding CD8<jats:sup>+<\/jats:sup> T cells. We quantified the sensitivity of these K<jats:sup>b<\/jats:sup>\u2010Ig microarrays by measuring a lower detection limit of 0.05% antigen\u2010specific CD8<jats:sup>+<\/jats:sup> T cells mixed with splenocytes from C57BL\/6J mouse. A fivefold increase in this lower detection limit (0.01%) was achieved using a secondary capture anti\u2010Ig antibody to coat the microarray surface. This higher sensitivity is comparable to that obtained using standard state\u2010of\u2010the\u2010art fluorescence activated cell sorting (FACS) instruments. We also found that contacting the T\u2010cell suspension with the K<jats:sup>b<\/jats:sup>\u2010Ig microarrays under mild shear flow conditions produced more uniform distributions of captured T cells on the individual spots and better spot\u2010to\u2010spot reproducibility across the entire microarray. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jmr.805","type":"journal-article","created":{"date-parts":[[2006,11,9]],"date-time":"2006-11-09T12:16:59Z","timestamp":1163074619000},"page":"32-38","source":"Crossref","is-referenced-by-count":23,"title":["Detection of antigen\u2010specific T cells on p\/MHC microarrays"],"prefix":"10.1002","volume":"20","author":[{"given":"Gokhan","family":"Deviren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kapil","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael E.","family":"Paulaitis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan P.","family":"Schneck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.01.146"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5284.94"},{"key":"e_1_2_1_4_1","first-page":"47","volume-title":"Transport Phenomena","author":"Bird RB","year":"1960"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.9.1395"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77291-7"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80161-2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.14.6671"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.7.3309"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(02)00203-X"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(00)00105-1"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.9.1633"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.6.2221"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.5.2427"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.6.1776"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1999.1441"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80470-7"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5443.1377"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.5.2555"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.125.4.945"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820130009062300"},{"key":"e_1_2_1_22_1","first-page":"17.2.1","volume-title":"Current Protocols in Immunology","author":"Schneck JP","year":"2000"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0000065"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407019102"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.4049\/jimmunol.162.3.1827","article-title":"A re\u2010evaluation of the frequency of CD8+ T cells specific for EBV in healthy virus carriers","volume":"162","author":"Tan LC","year":"1999","journal-title":"J. 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