{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T00:16:34Z","timestamp":1700093794416},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,3,26]],"date-time":"2008-03-26T00:00:00Z","timestamp":1206489600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Molecular Recognition"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human transferrin (hTf) is an 80\u2009kDa glycoprotein involved in iron transport from the absorption sites to the sites of storage and utilization. Additionally, transferrin also plays a relevant role as a bacteriostatic agent preventing uncontrolled bacterial growth in the host. In this work we describe a well\u2010characterized Mabs panel in terms of precise epitope localization and estimate affinity for the two major hTf isoforms. We found at least four antigenic regions in the hTf molecule, narrowed down the interacting antigen residues within three of such regions, and located them on a molecular model of hTf. Two of the antigenic regions partially overlap with previously described transferrin\u2010binding sites for both human receptor (antigenic region I: containing amino acid residues from Asp\u201069 to Asn\u201076 at the N\u2010lobe) and bacterial receptors from two pathogenic species (antigenic region III: amino acid residues from Leu\u2010665 to Ser\u2010672 at the C\u2010lobe). Hence, such monoclonal antibodies (Mabs) could be used as an additional tool for conformational studies and\/or the characterization of the interaction between hTf and both types of receptor molecules. Copyright \u00a9 2008 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jmr.878","type":"journal-article","created":{"date-parts":[[2008,3,26]],"date-time":"2008-03-26T18:54:40Z","timestamp":1206557680000},"page":"103-113","source":"Crossref","is-referenced-by-count":2,"title":["Epitope mapping of anti\u2010human transferrin monoclonal antibodies: potential uses for transferrin\u2013transferrin receptor interaction studies"],"prefix":"10.1002","volume":"21","author":[{"given":"Yasser","family":"Perera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dari\u00e9n","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osmany","family":"Guirola","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian","family":"Huerta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanet","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasmiana","family":"Mu\u00f1oz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,3,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.49.070180.002041"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01658.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0162-0134(92)84061-Q"},{"key":"e_1_2_1_5_1","first-page":"137","article-title":"Production and characterization of monoclonal antibodies against human transferrin","volume":"30","author":"Bartek J","year":"1984","journal-title":"Folia. 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