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These dsCT\u2010DNA\u2010PANI\u2010ClO<jats:sub>4<\/jats:sub>\/ITO and PANI\u2010ClO<jats:sub>4<\/jats:sub>\/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, scanning electron microscopy (SEM) and Fourier\u2010transform\u2010infrared (FTIR) measurements. This disposable dsCT\u2010DNA\u2010PANI\u2010ClO<jats:sub>4<\/jats:sub>\/ITO bioelectrode, stable for about 4 months, can be used to detect cypermethrin (0.005\u2009ppm) and trichlorfon (0.01\u2009ppm) in 30 and 60\u2009s, respectively. 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