{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:05:24Z","timestamp":1791133524933,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,6,13]],"date-time":"2003-06-13T00:00:00Z","timestamp":1055462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Magnetic Resonance Imaging"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To investigate the ability of magnetic resonance (MR) to monitor radio\u2010frequency (RF) ablation treatments by comparing MR images of thermal lesions to histologically assayed cellular damage. We developed a new methodology using three\u2010dimensional registration for making spatial correlations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A low\u2010field, open MRI system was used to guide an ablation probe into rabbit thigh muscle and acquire MR volumes after ablation. After fixation, we sliced and photographed the tissue at 3\u2010mm intervals, using a specially designed apparatus, to obtain a volume of tissue images. Histologic samples were digitized using a video microscopy system. For our three\u2010dimensional registration method, we used the tissue images as the reference, and registered histology and MR images to them using two different computer alignment steps. First, the MR volume was aligned to the volume of tissue images by registering needle fiducials placed near the tissue of interest. Second, we registered the histology images with the tissue images using a two\u2010dimensional warping technique that aligned internal features and the outside boundary of histology and tissue images.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The MR and histology images were very well aligned, and registration accuracy, determined from displacement of needle fiducials, was 1.32 \u00b1 0.39 mm (mean \u00b1 SD), which compared favorably to the MR voxel dimensions (0.70 mm in\u2010plane and 3.0 mm thick). A preliminary comparison of MR and tissue response showed that the region inside the elliptical hyperintense rim in MR closely corresponds to the region of necrosis as established by histology, with a mean absolute distance between MR and histology boundaries of 1.17 mm, slightly smaller than the mean registration error. The MR region slightly overestimated the region of necrosis, with a mean signed distance between boundaries of 0.85 mm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggest that our methodology can be used to achieve three\u2010dimensional registration of histology and in vivo MR images. In MR lesion images, the inner border of the hyperintense region corresponds to the border of irreversible cell damage. This is good evidence that during RF ablation treatments, iMRI lesion images can be used for real\u2010time feedback. J. Magn. Reson. Imaging 2003;18:90\u2013102. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmri.10324","type":"journal-article","created":{"date-parts":[[2003,6,16]],"date-time":"2003-06-16T10:13:16Z","timestamp":1055758396000},"page":"90-102","source":"Crossref","is-referenced-by-count":36,"title":["Three\u2010dimensional method for comparing in vivo interventional MR images of thermally ablated tissue with tissue response"],"prefix":"10.1002","volume":"18","author":[{"given":"Michael S.","family":"Breen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanya L.","family":"Lancaster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roee S.","family":"Lazebnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherif G.","family":"Nour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan S.","family":"Lewin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David L.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,6,13]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.168.1.3380968"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.1880080119"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.213.2.r99nv21461"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.1880080115"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.1880080114"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.1880080112"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2586(200101)13:1<23::AID-JMRI1004>3.0.CO;2-G"},{"key":"e_1_2_6_9_2","first-page":"294","article-title":"Comparison of SPECT and PET imaging for prostate cancer with histological correlation","volume":"42","author":"Lee Z","year":"2001","journal-title":"J Nucl Med"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1109\/TMI.2002.806270"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1109\/34.121791"},{"key":"e_1_2_6_12_2","article-title":"Volume registration using needle paths and point landmarks for evaluation of interventional MRI treatments","author":"Lazebnik RS","journal-title":"IEEE Trans Med Imaging"},{"key":"e_1_2_6_13_2","article-title":"Correcting spatial distortion of histological images","author":"Lancaster TL","journal-title":"Ann Biomed Eng"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.2214\/ajr.166.6.8633445"},{"key":"e_1_2_6_15_2","volume-title":"Atlas of the visible human male: reverse engineering of the human body","author":"Spitzer VM","year":"1998"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-6111(96)00072-9"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(94)90196-1"},{"key":"e_1_2_6_18_2","first-page":"820","article-title":"A high resolution digital collection of cryosectioned whole brain","volume":"13","author":"Toga AW","year":"1993","journal-title":"J Cereb Blood Flow Metab"},{"key":"e_1_2_6_19_2","first-page":"75","article-title":"Shrinkage of muscle fibres during the fixation of cadaveric tissue","volume":"160","author":"Cutts A","year":"1988","journal-title":"J Anat"},{"key":"e_1_2_6_20_2","first-page":"202","volume-title":"Myocardial cell death, including ischemic heart disease and its complications. 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