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Drugs were administered topically by mixing them with the water used for stomach washing, and a series of images was collected. In untreated animals, a mucous layer emitting a high intensity signal covered the gastric mucosa. Atropine administration strongly reduced the mucous layer thickness. After pilocarpine administration, MRI images showed that the mucous layer was thicker than in controls. After metacholine administration, the thickness of the mucous layer was increased in comparison with both controls and pilocarpine\u2010treated animals, in accord with the expected drug\u2010induced increase of mucous secretion. Quantitative analysis of the mucous layer thickness on MRI images confirmed the above findings. In conclusion, these results indicate that MRI at 7 T is a sensitive in vivo screening test to evaluate the effect of drugs on gastric mucus secretion in absence of surgical manipulation, thereby enabling morphofunctional studies that cannot be performed by conventional methods.<\/jats:p>","DOI":"10.1002\/jmri.1880060213","type":"journal-article","created":{"date-parts":[[2007,3,16]],"date-time":"2007-03-16T06:16:46Z","timestamp":1174025806000},"page":"336-340","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of frog gastric mucous secretion by 7T magnetic resonance imaging"],"prefix":"10.1002","volume":"6","author":[{"given":"Andrea","family":"Sbarbati","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7oise","family":"Leclercq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Zancanaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uliano","family":"Guerrini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kostas","family":"Antonakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Osculati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"G561","article-title":"Gastric mucosal barrier: hydrophobic lining to the lumen of the stomach","volume":"244","author":"Hills BA","year":"1983","journal-title":"Am J Physiol"},{"key":"e_1_2_1_3_2","first-page":"G342","article-title":"Gastric mucosal barrier: stabilization of hydrophobic lining to the stomach mucus","volume":"249","author":"Hills BA","year":"1985","journal-title":"Am J Physiol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6828859"},{"key":"e_1_2_1_5_2","first-page":"617","volume-title":"Physiology of the Gastrointestinal Tract","author":"Allen A","year":"1981"},{"key":"e_1_2_1_6_2","first-page":"27","volume-title":"Gastric Secretion. 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