{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T20:50:10Z","timestamp":1771015810762,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,12,18]],"date-time":"2008-12-18T00:00:00Z","timestamp":1229558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Magnetic Resonance Imaging"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To evaluate manganese (Mn<jats:sup>2+<\/jats:sup>)\u2010enhanced MRI (MEMRI) and diffusion tensor imaging (DTI) as tools for detection of axonal injury and regeneration after intravitreal peripheral nerve graft (PNG) implantation in the rat optic nerve (ON).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>In adult Fischer rats, retinal ganglion cell (RGC) survival was evaluated in Flurogold (FG) back\u2010filled retinal whole mounts after ON crush (ONC), intravitreal PNG, and intravitreal MnCl<jats:sub>2<\/jats:sub> injection (150 nmol) at 0 and 20 days post lesion (dpl). MEMRI and echo\u2010planar DTI (DTI\u2010EPI) was obtained of noninjured ON one day after intravitreal MnCl<jats:sub>2<\/jats:sub> injection, and at 1 and 21 dpl after ONC, intravitreal PNG, and intravitreal MnCl<jats:sub>2<\/jats:sub> injections given at 0 and 20 dpl. GAP\u201043 immunohistochemistry was performed after the last MRI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ONC reduced RGC density in retina by 94% at 21 dpl compared to noninjured ON without MnCl<jats:sub>2<\/jats:sub> injections. Both intravitreal PNG and intravitreal MnCl<jats:sub>2<\/jats:sub> injections improved RGC survival in retina, which was reduced by 90% (ONC+MnCl<jats:sub>2<\/jats:sub>), 82% (ONC+PNG), and 74% (ONC+PNG+MnCl<jats:sub>2<\/jats:sub>) compared to noninjured ON. DTI\u2010derived parameters (fractional anisotropy [FA], mean diffusivity, axial diffusivity \u03bb<jats:sub>\u2225<\/jats:sub>, and radial diffusivity \u03bb<jats:sub>\u27c2<\/jats:sub>) were unaffected by the presence of Mn<jats:sup>2+<\/jats:sup> in the ON. At 1 dpl, CNR<jats:sub>MEMRI<\/jats:sub> and \u03bb<jats:sub>\u2225<\/jats:sub> were reduced at the injury site, while at 21 dpl they were increased at the injury site compared to values measured at 1 dpl. GAP\u201043 immunoreactive axons were present in the ON distal to the ONC injury site.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MEMRI and DTI enabled detection of functional and structural degradation after rat ON injury, and there was correlation between the MRI\u2010derived and immunohistochemical measures of axon regeneration. J. Magn. Reson. Imaging 2009;29:39\u201351. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmri.21606","type":"journal-article","created":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T18:33:00Z","timestamp":1230143580000},"page":"39-51","source":"Crossref","is-referenced-by-count":46,"title":["Combination of Mn<sup>2+<\/sup>\u2010enhanced and diffusion tensor MR imaging gives complementary information about injury and regeneration in the adult rat optic nerve"],"prefix":"10.1002","volume":"29","author":[{"given":"Marte","family":"Thuen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d8ystein","family":"Olsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Berry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina 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