{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T01:55:41Z","timestamp":1768096541124,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,10,28]],"date-time":"2010-10-28T00:00:00Z","timestamp":1288224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Magnetic Resonance Imaging"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To investigate in vivo MRI tracking mesenchymal stem cells (MSCs) in peripheral nerve injures using a clinically available paramagnetic contrast agent (Gd\u2010DTPA) and commercially available rhodamine\u2010incorporated transfection reagents (PEI\u2010FluoR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>After bone marrow MSCs were labeled with Gd\u2010DTPA and PEI\u2010FluoR complex, the labeling efficacy and longevity of Gd\u2010DTPA maintenance were measured and cell viability, proliferation, and apoptosis were assessed. Thirty\u2010six rabbits with acute sciatic nerve traction injury randomly received 1 \u00d7 10<jats:sup>6<\/jats:sup> labeled (n = 12) or unlabeled MSCs (n = 12) or vehicle alone injection. The distribution and migration of implanted cells was followed by MRI and correlated with histology. The relative signal intensity (RSL) of the grafts was measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The labeling efficiency was 76 \u00b1 4.7% and the labeling procedure did not in\ufb02uence cell viability, proliferation, and apoptosis. A persistent higher RSL in grafts was found in the labeled group compared with the unlabeled and vehicle groups until 10 days after transplantation (<jats:italic>P<\/jats:italic> &lt; 0.05). The distribution and migration of labeled cells could be tracked by MRI until 10 days after transplantation. Transplanted MSCs were not found to transdifferentiate into Schwann\u2010like cells within 14\u2010day follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Labeling MSCs with the dual agents may enable cellular MRI of the engraftment in the experimental peripheral nerve injury. J. Magn. Reson. Imaging 2010;32:1076\u20131085. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmri.22353","type":"journal-article","created":{"date-parts":[[2010,10,29]],"date-time":"2010-10-29T11:55:26Z","timestamp":1288353326000},"page":"1076-1085","source":"Crossref","is-referenced-by-count":26,"title":["In vivo MR imaging tracking of transplanted mesenchymal stem cells in a rabbit model of acute peripheral nerve traction 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